CD74, MHC CLASS11 INVARIANT CHAIN anticorps

N° du produit ABIN965816

Détail du produit

Antigène: CD74, MHC Class 11 invariant Chain

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Specificité: This antibody reacts with several isoforms of the CD74 antigen, the invariant chain of HLA-DR.

Purification: Purified.

Immunogène: Cell extracts from diffuse histolytic lymphoma SU-DHL-4 cells.

Clone: B318 (LN-2)

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 10-20 ug/mL on frozen or formalin fixed paraffin embedded tissue sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results

Commentaires:

Recommended Positive Control: Tonsil

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détail du antigène

Antigène: CD74, MHC Class 11 invariant Chain

Autre désignation: CD74,MHC Class11 Invariant Chain

ID gène: 972