CD8 anticorps

N° du produit ABIN965819

L’anticorps Souris Monoclonal anti-CD8 a été validé pour FACS, IF, IHC (fro), ELISA et IHC (af). Il convient pour détecter CD8 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour CD8 anticorps (ABIN965819)

Antigène: CD8

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Acetone-fixed) (IHC (af))

Clone: B362 (RIV 11)

Détail du produit anti-CD8 anticorps

Specificité: This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen.

Purification: Purified.

Immunogène: Human peripheral lymphocytes.

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
ELISA: 0.2-1.0 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

Commentaires:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-CD8 (ABIN965819)

Barber, Dasgupta, Schlossman, Trevillyan, Rudd: "The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 9, pp. 3277-81, (1989) (PubMed).

Jonker, Nooij, den Butter, van Lambalgen, Fuccello: "Side effects and immunogenicity of murine lymphocyte-specific monoclonal antibodies in subhuman primates." dans: Transplantation, Vol. 45, Issue 4, pp. 677-82, (1988) (PubMed).

Reinherz, Kung, Goldstein, Schlossman: "A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 3, pp. 1301-7, (1980) (PubMed).

Détails sur CD8

Antigène: CD8

ID gène: 925