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GFP anticorps

GFP Reactivité: Aequorea victoria WB, ELISA, IF, FACS Hôte: Souris Monoclonal 4B10B2 unconjugated
N° du produit ABIN966200
  • Antigène Voir toutes GFP Anticorps
    GFP (Green Fluorescent Protein (GFP))
    Reactivité
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    • 19
    • 15
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    Aequorea victoria
    Hôte
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    Souris
    Clonalité
    • 123
    • 89
    Monoclonal
    Conjugué
    • 106
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    Cet anticorp GFP est non-conjugé
    Application
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    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purification
    Purified
    Immunogène
    Purified recombinant fragment of GFP expressed in E. Coli.
    Clone
    4B10B2
    Isotype
    IgG2a
    Top Product
    Discover our top product GFP Anticorps primaire
  • Indications d'application
    Western Bloting: 1/500 - 1/2000.
    Immunofluorescence: 1/200 - 1/1000.
    Flow cytometry: 1/200 - 1/400.
    ELISA: Propose dilution 1/10000.
    Not yet tested in other applications.
    Determining optimal working dilutions by titration test.
    Restrictions
    For Research Use only
  • Buffer
    PBS
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
  • Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, (2006) (PubMed).

    Cavalieri, Bernardo, Spinelli: "Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells." dans: Gene expression patterns : GEP, Vol. 7, Issue 1-2, pp. 124-30, (2006) (PubMed).

  • Antigène
    GFP (Green Fluorescent Protein (GFP))
    Autre désignation
    GFP (GFP Produits)
    Synonymes
    anticorps green fluorescent protein, anticorps gfp
    Classe de substances
    Viral Protein
    Sujet
    GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
    Poids moléculaire
    27kDa
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