GST anticorps

N° du produit ABIN966238

Cet anticorps Monoclonal détecte spécifiquement GST dans WB et ELISA. Il présente une réactivité envers Schistosoma japonicum et a été mentionné dans 1 publication.

Aperçu rapide pour GST anticorps (ABIN966238)

Antigène: GST (Glutathione S Transferase (GST))

Reactivité: Schistosoma japonicum

Hôte: Veuillez nous consulter SVP

Clonalité: Monoclonal

Conjugué: Cet anticorp GST est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-GST anticorps

Specificité: Ni-NTA purified recombinant GST expressed in E. Coli strain M15.

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Références pour anticorps anti-GST (ABIN966238)

Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." dans: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).

Détails sur GST

Antigène: GST (Glutathione S Transferase (GST))

Autre désignation: Glutathione-S-Transferase (GST)

Sujet: Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.