NPT anticorps

N° du produit ABIN966694

Détail du produit

Antigène: NPT

Reactivité: Humain

Hôte: Veuillez nous consulter SVP

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Specificité: Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3)

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG2a

Information d'application

Indications d'application: Western Blot: Dilution 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Référence

Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." dans: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).

Détail du antigène

Antigène: NPT

Sujet: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.