NPT anticorps

Détails pour le produit réf. ABIN966694, Fournisseur: Connectez-vous pour afficher
Antigène
Reactivité
Humain
9
Hôte
9
Clonalité
Monoclonal
Application
ELISA, Western Blotting (WB)
9
7
1
Options
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N° du produit (Fournisseur)
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Isotype IgG2a
Specificité Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3)
Purification Antibodies are purified by protein A affinity chromatography
Sujet Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
Indications d'application Western Blot: Dilution 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Stock -20 °C
Produit citée dans: Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." dans: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, 2001 (PubMed).

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