Vimentin anticorps

N° du produit ABIN967252

Cet anticorps Souris Monoclonal détecte spécifiquement Vimentin dans WB, IHC et ELISA. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Vimentin anticorps (ABIN967252)

Antigène: Vimentin (VIM)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 9E7E7-5G3F10-5G3F10E5

Détail du produit anti-Vimentin anticorps

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of Vimentin expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-Vimentin (ABIN967252)

Vanzi: "Freedom at home: state constitutions and Medicaid funding for abortions." dans: New Mexico law review, Vol. 26, Issue 3, pp. 433-54, (2005) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms: FLJ36605, VIM

ID gène: 7431

Pathways: Caspase Cascade in Apoptosis