MHC, Class I anticorps

N° du produit ABIN967374

Cet anticorps anti-MHC, Class I est un anticorps Souris Monoclonal détectant MHC, Class I dans FACS, WB, IP, ELISA et IHC (f). Adapté pour Souris. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour MHC, Class I anticorps (ABIN967374)

Antigène: MHC, Class I

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC, Class I est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SF1-1-1

Détail du produit anti-MHC, Class I anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: BALB/c mouse cells

Isotype: IgG2a kappa

Information d'application

Indications d'application: For immunohistochemical staining (IHC) of acetone-fixed frozen sections, we recommend the use of biotinylated SF1-1.1 mAb.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-MHC, Class I (ABIN967374)

Noun, Reboul, Abastado, Jaulin, Kourilsky, Pla: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, (1996) (PubMed).

Zhao, Iwata: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." dans: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).

Abastado, Casrouge, Kourilsky: "Differential role of conserved and polymorphic residues of the binding groove of MHC class I molecules in the selection of peptides." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3569-75, (1993) (PubMed).

Détails sur MHC, Class I

Antigène: MHC, Class I

Autre désignation: H-2K d

Sujet: The SF1-1.1 antibody reacts with the alpha3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g, b, j, k, p, q, s, v) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e, ABIN967568 and ABIN967370). This antibody is routinely tested by flow cytometric analysis.