MADCAM1 anticorps

N° du produit ABIN967383

Cet anticorps anti-MADCAM1 est un anticorps Rat Monoclonal détectant MADCAM1 dans WB, FACS, IHC (fro), IP, IAC et BR. Adapté pour Souris. Ce Primary Antibody a été cité dans 9+ publications.

Aperçu rapide pour MADCAM1 anticorps (ABIN967383)

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp MADCAM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunoaffinity Chromatography (IAC), Blocking Reagent (BR)

Clone: MECA-367

Détail du produit anti-MADCAM1 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.

Isotype: IgG2a kappa

Information d'application

Indications d'application: For IHC, we recommend the use of purified MECA-367 mAb in our special formulation for immunohistochemistry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-MADCAM1 (ABIN967383)

Yang, Sytwu, McDevitt, Michie: "Involvement of beta 7 integrin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the development of diabetes in obese diabetic mice." dans: Diabetes, Vol. 46, Issue 10, pp. 1542-7, (1997) (PubMed).

Hänninen, Salmi, Simell, Andrew, Jalkanen: "Recirculation and homing of lymphocyte subsets: dual homing specificity of beta 7-integrin(high)-lymphocytes in nonobese diabetic mice." dans: Blood, Vol. 88, Issue 3, pp. 934-44, (1996) (PubMed).

Kraal, Schornagel, Streeter, Holzmann, Butcher: "Expression of the mucosal vascular addressin, MAdCAM-1, on sinus-lining cells in the spleen." dans: The American journal of pathology, Vol. 147, Issue 3, pp. 763-71, (1995) (PubMed).

Berg, McEvoy, Berlin, Bargatze, Butcher: "L-selectin-mediated lymphocyte rolling on MAdCAM-1." dans: Nature, Vol. 366, Issue 6456, pp. 695-8, (1994) (PubMed).

Hamann, Andrew, Jablonski-Westrich, Holzmann, Butcher: "Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3282-93, (1994) (PubMed).

Lee, Sarvetnick: "Induction of vascular addressins and adhesion molecules in the pancreas of IFN-gamma transgenic mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 9, pp. 4597-603, (1994) (PubMed).

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." dans: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

Briskin, McEvoy, Butcher: "MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1." dans: Nature, Vol. 363, Issue 6428, pp. 461-4, (1993) (PubMed).

Streeter, Berg, Rouse, Bargatze, Butcher: "A tissue-specific endothelial cell molecule involved in lymphocyte homing." dans: Nature, Vol. 331, Issue 6151, pp. 41-6, (1988) (PubMed).

Détails sur MADCAM1

Antigène: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Autre désignation: MAdCAM-1

Sujet: The MECA-367 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-gamma can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin alpha4beta7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer’s patch HEV in vivo. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.