L1 Protein anticorps

N° du produit ABIN967423

Cet anticorps Souris Monoclonal détecte spécifiquement L1 Protein dans IHC (f), IF et WB. Il présente une réactivité avec des échantillons de Papillomavirus. Il a été cité dans 4+ publications.

Aperçu rapide pour L1 Protein anticorps (ABIN967423)

Antigène: L1 Protein

Reactivité: Papillomavirus

Hôte: Souris

Clonalité: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Western Blotting (WB)

Clone: CAMVIR

Détail du produit

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Référence

Doorbar, Ely, Coleman, Hibma, Davies, Crawford: "Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein." dans: Virology, Vol. 187, Issue 1, pp. 353-9, (1992) (PubMed).

Zhou, Sun, Stenzel, Frazer: "Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles." dans: Virology, Vol. 185, Issue 1, pp. 251-7, (1991) (PubMed).

McLean, Churcher, Meinke, Smith, Higgins, Stanley, Minson: "Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus." dans: Journal of clinical pathology, Vol. 43, Issue 6, pp. 488-92, (1990) (PubMed).

de Villiers: "Heterogeneity of the human papillomavirus group." dans: Journal of virology, Vol. 63, Issue 11, pp. 4898-903, (1989) (PubMed).

Détail du antigène

Antigène: L1 Protein

Classe de substances: Viral Protein

Sujet: More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.