HSP70 anticorps

N° du produit ABIN967447

Cet anticorps Souris Monoclonal détecte spécifiquement HSP70 dans WB et IP. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour HSP70 anticorps (ABIN967447)

Antigène: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP70 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 5G10

Détail du produit anti-HSP70 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified Hsp70

Isotype: IgG2b

Information d'application

Indications d'application: HeLa cervical carcinoma cells (ATCC CCL-2) are suggested as a positive control.

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-HSP70 (ABIN967447)

Cyr, Langer, Douglas: "DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70." dans: Trends in biochemical sciences, Vol. 19, Issue 4, pp. 176-81, (1994) (PubMed).

Craig, Gross: "Is hsp70 the cellular thermometer?" dans: Trends in biochemical sciences, Vol. 16, Issue 4, pp. 135-40, (1991) (PubMed).

Singh, Yu: "Accumulation of a heat shock-like protein during differentiation of human erythroid cell line K562." dans: Nature, Vol. 309, Issue 5969, pp. 631-3, (1984) (PubMed).

Détails sur HSP70

Antigène: HSP70 (Heat Shock Protein 70 (HSP70))

Autre désignation: Hsp70

Sujet: Cells from many organisms increase the expression of heat shock or stress proteins in response to adverse changes. The heat shock protein (HSP) 70 family consists of multiple members, localized within different intracellular compartments. HSP 70 is constitutively expressed in many human cells with increased expression observed following stress. Members of the HSP 70 family bind ATP, and are thought to function as molecular chaperones by assisting in the folding of other proteins in various intracellular compartments. HSP 70 migrates at a molecular weight of 70 kDa in SDS-PAGE. The 5G10 antibody recognizes human HSP 70. Purified HSP 70 was isolated from K562, a human erythroid cell line, and used as immunogen.

Poids moléculaire: 70 kDa