FLI1 anticorps

N° du produit ABIN967451

L’anticorps Souris Monoclonal anti-FLI1 a été validé pour WB et IP. Il convient pour détecter FLI1 dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour FLI1 anticorps (ABIN967451)

Antigène: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FLI1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: G146-254

Détail du produit anti-FLI1 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Fli-1 ets Domain Fusion Protein

Isotype: IgG2a

Information d'application

Indications d'application: Applications include western blot analysis (0.5-2 µg/ml). Additional applications not routinely tested include gel shift and immunoprecipitation. Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. In gel shift assays this antibody supershifts complexes of Fli-1 and DNA probe.

Commentaires:

Related Products: ABIN967389, ABIN968537

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-FLI1 (ABIN967451)

Bailly, Bosselut, Zucman, Cormier, Delattre, Roussel, Thomas, Ghysdael: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." dans: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).

May, Lessnick, Braun, Klemsz, Lewis, Lunsford, Hromas, Denny: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." dans: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, (1994) (PubMed).

Détails sur FLI1

Antigène: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Autre désignation: Fli-1

Sujet: Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through a 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma through at (11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli-1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to trans-activate the promoter for c-myc. C-myc is known to be elevated in Ewing sarcomas. Clone G146-254 was raised against a bacterially expressed Fli-1 ets domain fusion protein.

Poids moléculaire: 50 kDa