Sca-1/Ly-6A/E anticorps

N° du produit ABIN967571

Détail du produit anti-Sca-1/Ly-6A/E anticorps

Antigène: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Sca-1/Ly-6A/E est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: IL-2-dependent mouse T-cell line CTL-L

Clone: D7

Isotype: IgG2a kappa

Information d'application

Commentaires:

Related Products: ABIN967571

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-Sca-1/Ly-6A/E (ABIN967571)

Yonemura, Ku, Lyman, Ogawa: "In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand." dans: Blood, Vol. 89, Issue 6, pp. 1915-21, (1997) (PubMed).

Ito, Anan, Misawa, Kai, Hara: "In vitro differentiation of murine Sca-1+Lin- cells into myeloid, B cell and T cell lineages." dans: Stem cells (Dayton, Ohio), Vol. 14, Issue 4, pp. 412-8, (1996) (PubMed).

Moore, Bennett, Kumar: "Transplantable NK cell progenitors in murine bone marrow." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1653-63, (1995) (PubMed).

Fleming, Malek: "Multiple glycosylphosphatidylinositol-anchored Ly-6 molecules and transmembrane Ly-6E mediate inhibition of IL-2 production." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 5, pp. 1955-62, (1994) (PubMed).

Flood, Dougherty, Ron: "Inhibition of Ly-6A antigen expression prevents T cell activation." dans: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 115-20, (1990) (PubMed).

Codias, Malek: "Regulation of B lymphocyte responses to IL-4 and IFN-gamma by activation through Ly-6A/E molecules." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 6, pp. 2197-204, (1990) (PubMed).

Codias, Rutter, Fleming, Malek: "Down-regulation of IL-2 production by activation of T cells through Ly-6A/E." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1407-14, (1990) (PubMed).

Codias, Cray, Baler, Levy, Malek: "Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes." dans: Immunogenetics, Vol. 29, Issue 2, pp. 98-107, (1989) (PubMed).

Malek, Danis, Codias: "Tumor necrosis factor synergistically acts with IFN-gamma to regulate Ly-6A/E expression in T lymphocytes, thymocytes and bone marrow cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 6, pp. 1929-36, (1989) (PubMed).

Malek, Ortega, Chan, Kroczek, Shevach: "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies." dans: The Journal of experimental medicine, Vol. 164, Issue 3, pp. 709-22, (1986) (PubMed).

Ortega, Korty, Shevach, Malek: "Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 10, pp. 3240-6, (1986) (PubMed).

Palfree, Dumont, Hammerling: "Ly-6A.2 and Ly-6E.1 molecules are antithetical and identical to MALA-1." dans: Immunogenetics, Vol. 23, Issue 3, pp. 197-207, (1986) (PubMed).

Détails sur Sca-1/Ly-6A/E

Antigène: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Autre désignation: Ly-6A/E (Ly6a Produits)

Synonymes: anticorps Ly-6A.2, anticorps Ly-6A/E, anticorps Ly-6E.1, anticorps Sca-1, anticorps Sca1, anticorps TAP, anticorps lymphocyte antigen 6 complex, locus A, anticorps Ly6a

Sujet: The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E) can block binding of FITC-conjugated D7 antibody to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody. Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel to identify HSC. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Sca-1

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation