DPP4 anticorps

N° du produit ABIN967645

Détail du produit anti-DPP4 anticorps

Antigène: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DPP4 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat dendritic cells enriched from thoracic duct lymph

Clone: OX-61

Isotype: IgG2a kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-DPP4 (ABIN967645)

Iwaki-Egawa, Watanabe, Fujimoto: "CD26/dipeptidyl peptidase IV does not work as an adenosine deaminase-binding protein in rat cells." dans: Cellular immunology, Vol. 178, Issue 2, pp. 180-6, (1997) (PubMed).

Johnson, Zhu, Augustin-Voss, Pauli: "Lung endothelial dipeptidyl peptidase IV is an adhesion molecule for lung-metastatic rat breast and prostate carcinoma cells." dans: The Journal of cell biology, Vol. 121, Issue 6, pp. 1423-32, (1993) (PubMed).

Kameoka, Tanaka, Nojima, Schlossman, Morimoto: "Direct association of adenosine deaminase with a T cell activation antigen, CD26." dans: Science (New York, N.Y.), Vol. 261, Issue 5120, pp. 466-9, (1993) (PubMed).

Bristol, Sakaguchi, Appella, Doyle, Takács: "Thymocyte costimulating antigen is CD26 (dipeptidyl-peptidase IV). Costimulation of granulocyte, macrophage, and T lineage cell proliferation via CD26." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 367-72, (1992) (PubMed).

Gorrell, Wickson, McCaughan: "Expression of the rat CD26 antigen (dipeptidyl peptidase IV) on subpopulations of rat lymphocytes." dans: Cellular immunology, Vol. 134, Issue 1, pp. 205-15, (1991) (PubMed).

McCaughan, Wickson, Creswick, Gorrell: "Identification of the bile canalicular cell surface molecule GP110 as the ectopeptidase dipeptidyl peptidase IV: an analysis by tissue distribution, purification and N-terminal amino acid sequence." dans: Hepatology (Baltimore, Md.), Vol. 11, Issue 4, pp. 534-44, (1990) (PubMed).

Piazza, Callanan, Mowery, Hixson: "Evidence for a role of dipeptidyl peptidase IV in fibronectin-mediated interactions of hepatocytes with extracellular matrix." dans: The Biochemical journal, Vol. 262, Issue 1, pp. 327-34, (1989) (PubMed).

Hanski, Huhle, Gossrau, Reutter: "Direct evidence for the binding of rat liver DPP IV to collagen in vitro." dans: Experimental cell research, Vol. 178, Issue 1, pp. 64-72, (1988) (PubMed).

Détails sur DPP4

Antigène: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Autre désignation: CD26 (DPP4 Produits)

Synonymes: anticorps ADABP, anticorps ADCP2, anticorps CD26, anticorps DPPIV, anticorps TP103, anticorps MGC81966, anticorps MOP9.8, anticorps MOP9_8, anticorps DPP4, anticorps si:ch73-2d23.3, anticorps Cd26, anticorps Dpp-4, anticorps THAM, anticorps dipeptidyl peptidase 4, anticorps dipeptidyl-peptidase 4 S homeolog, anticorps prolyl oligopeptidase family protein, anticorps dipeptidyl-peptidase 4, anticorps dipeptidylpeptidase 4, anticorps DPP4, anticorps dpp4.S, anticorps AT5G24260, anticorps dpp4, anticorps Dpp4

Sujet: The OX-61 antibody reacts with a type II transmembrane glycoprotein, CD26, which is a serine exoprotease identified as dipeptidyl/peptidase IV. Among various biological activities, rat CD26 binds fibronectin and collagen. Rat CD26 is involved in the costimulation of thymocyte proliferation in vitro, particularly the CD4-/CD8- subset, and is developmentally regulated on hematopoietic cells. Although mouse and human CD26 anchor ADA (adenosine deaminase) to cell membranes, rat CD26 does not function as an ADA-binding protein. Rat CD26 is expressed in lung endothelial cells, as well as in various epithelial cells. T cells express lower levels of CD26 than CD4-/CD8- thymocytes. The distribution of CD26 antigen in rat bone marrow cells is similar to that of human CD26. OX-61 monoclonal antibody stains CD4+, CD8+, and Ig+ lymphocytes, and the staining increases upon activation.

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity