IFNGR1 anticorps

N° du produit ABIN967652

L’anticorps Hamster arménien Monoclonal anti-IFNGR1 a été validé pour FACS et IP. Il convient pour détecter IFNGR1 dans des échantillons de Souris. Il y a 1 publication disponible.

Aperçu rapide pour IFNGR1 anticorps (ABIN967652)

Antigène: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp IFNGR1 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 2E2

Détail du produit anti-IFNGR1 anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of 2E2 can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of IFN-gammaRalpha. An appropriate purified immunoglobulin isotype control is A19-3. A three-layer staining protocol is recommended for maximizing the detection IFN-gammaRalpha chains expressed by cells as detailed in the figure legend.
2E2 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the ligand (i.e., IFN-gamma) for IFN-gamma receptors is present. Immunoprecipitation: The 2E2 antibody has been reported to be useful for the immunoprecipitation of IFN-gammaRalpha chains from lysates of cloned mouse T cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-IFNGR1 (ABIN967652)

Bach, Szabo, Dighe, Ashkenazi, Aguet, Murphy, Schreiber: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." dans: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).

Détails sur IFNGR1

Antigène: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Autre désignation: CD119

Sujet: The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon-gamma receptor (IFN-gammaRalpha, aka, CD119). The functionally active-form of the mouse IFN-gamma receptor consists of two (or more) subunits, with IFN-gammaRalpha responsible for IFN-gamma binding and both the IFN-gammaRalpha and IFN-gammaRbeta chains required for the transduction of biologic responses. IFN-gammaRalpha is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody, it does not block the binding of IFN-gamma to its receptor. The immunogen used to generate this hybridoma was a purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein.
Synonyms: IFN-gamma Receptor alpha chain

Pathways: Interferon-gamma Pathway