Phosphotyrosine anticorps

N° du produit ABIN967685

Il existe 6+ publications pour ce produit. L’anticorps anti-Phosphotyrosine Monoclonal Souris est utilisé pour la détection de Phosphotyrosine. Il a été validé pour WB, IP, IF et IHC.

Aperçu rapide pour Phosphotyrosine anticorps (ABIN967685)

Antigène: Phosphotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: PY20

Détail du produit anti-Phosphotyrosine anticorps

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2b

Information d'application

Indications d'application: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.

Commentaires:

Related Products: ABIN968534, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing glycerol and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Phosphotyrosine (ABIN967685)

Yoon, Kovalenko, Bogdanov, Wallach: "MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation." dans: Immunity, Vol. 47, Issue 1, pp. 51-65.e7, (2017) (PubMed).

Yamakawa, Tsuchida, Sugino: "The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors." dans: The EMBO journal, Vol. 21, Issue 7, pp. 1684-94, (2002) (PubMed).

Baisden, Gatesman, Cherezova, Jiang, Flynn: "The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases." dans: Oncogene, Vol. 20, Issue 45, pp. 6607-16, (2001) (PubMed).

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." dans: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).

Vinós, Freeman: "Evidence that Argos is an antagonistic ligand of the EGF receptor." dans: Oncogene, Vol. 19, Issue 31, pp. 3560-2, (2000) (PubMed).

Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." dans: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Classe de substances: Amino Acid

Sujet: Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.