Phosphotyrosine anticorps (HRP)

N° du produit ABIN967694

Il existe 5+ publications pour ce produit. L’anticorps anti-Phosphotyrosine Monoclonal Souris est utilisé pour la détection de Phosphotyrosine. Il a été validé pour WB, IP, IF et IHC.

Aperçu rapide pour Phosphotyrosine anticorps (HRP) (ABIN967694)

Antigène: Phosphotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: PY20

Détail du produit anti-Phosphotyrosine anticorps (HRP)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2b

Information d'application

Indications d'application: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.

Commentaires:

Related Products: ABIN968534

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol.

Stock: -20 °C

Stockage commentaire: The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed by gel filtration chromatography. Store undiluted at -20° C.

Références pour anticorps anti-Phosphotyrosine (HRP) (ABIN967694)

Hemmeryckx, Reichert, Watanabe, Kaartinen, de Jong, Pattengale, Groffen, Heisterkamp: "BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl." dans: Oncogene, Vol. 21, Issue 20, pp. 3225-31, (2002) (PubMed).

Alderton, Rakhit, Kong, Palmer, Sambi, Pyne, Pyne: "Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells." dans: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28578-85, (2001) (PubMed).

Borges, Jan, Ruoslahti: "Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain." dans: The Journal of biological chemistry, Vol. 275, Issue 51, pp. 39867-73, (2001) (PubMed).

Goitsuka, Tatsuno, Ishiai, Kurosaki, Kitamura: "MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT." dans: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36043-50, (2001) (PubMed).

Smith, Surviladze, Gaudet, Backer, Mitchell, Wilson: "p110beta and p110delta phosphatidylinositol 3-kinases up-regulate Fc(epsilon)RI-activated Ca2+ influx by enhancing inositol 1,4,5-trisphosphate production." dans: The Journal of biological chemistry, Vol. 276, Issue 20, pp. 17213-20, (2001) (PubMed).

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Classe de substances: Amino Acid

Sujet: Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.