Fibronectin anticorps

N° du produit ABIN967729

Cet anticorps Souris Monoclonal détecte spécifiquement Fibronectin dans WB, IHC, IF et IP. Il présente une réactivité envers Humain, Rat, Souris, Boeuf (Vache), Poulet et Chien et a été mentionné dans 5+ publications.

Aperçu rapide pour Fibronectin anticorps (ABIN967729)

Antigène: Fibronectin

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 10-Fibronectin

Détail du produit anti-Fibronectin anticorps

Réactivité croisée: Boeuf (Vache), Poulet, Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Fibronectin

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Fibronectin (ABIN967729)

Danen, Sonneveld, Brakebusch, Fassler, Sonnenberg: "The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis." dans: The Journal of cell biology, Vol. 159, Issue 6, pp. 1071-86, (2002) (PubMed).

Rhee, Sen, Lu, Wu, Leoni, Rubin, Corr, Carson: "Wnt and frizzled receptors as potential targets for immunotherapy in head and neck squamous cell carcinomas." dans: Oncogene, Vol. 21, Issue 43, pp. 6598-605, (2002) (PubMed).

Zuk, Bonventre, Brown, Matlin: "Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney." dans: The American journal of physiology, Vol. 275, Issue 3 Pt 1, pp. C711-31, (1998) (PubMed).

Chen, Mosher: "Formation of sodium dodecyl sulfate-stable fibronectin multimers. Failure to detect products of thiol-disulfide exchange in cyanogen bromide or limited acid digests of stabilized matrix fibronectin." dans: The Journal of biological chemistry, Vol. 271, Issue 15, pp. 9084-9, (1996) (PubMed).

Sechler, Takada, Schwarzbauer: "Altered rate of fibronectin matrix assembly by deletion of the first type III repeats." dans: The Journal of cell biology, Vol. 134, Issue 2, pp. 573-83, (1996) (PubMed).

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: The 240 kDa dimeric fibronectin protein exists in two forms: a soluble protomer in body fluids and an insoluble multimer in the extracellular matrix. The latter is the primary functional form and creates a substrate for cell migration, a role which makes fibronectin vital to embryogenesis and wound response. Fibronectin mediates cytoskeletal organization, cell attachment, and cellular signaling through interactions with cellular receptors. Although various isoforms of fibronectin are derived by alternative splicing, they share a common N-terminus which is a critical region for cell surface binding in an initial step of multimer assembly. Further polymerization steps are regulated by fibronectin/integrin interactions and result in generation of the complex fibrils that constitute the fibronectin matrix.
Synonyms: FN, LETS

Poids moléculaire: 240 kDa