RBP4 anticorps (AA 1-135)

N° du produit ABIN967847

L’anticorps Souris Monoclonal anti-RBP4 a été validé pour WB, IHC, IP et IF. Il convient pour détecter RBP4 dans des échantillons de Humain, Souris, Rat, Poulet et Chien. Il y a 5+ publications disponibles.

Aperçu rapide pour RBP4 anticorps (AA 1-135) (ABIN967847)

Antigène: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reactivité: Humain, Souris, Rat, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RBP4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 42-RBP

Détail du produit anti-RBP4 anticorps

Épitope: AA 1-135

Réactivité croisée: Chien, Poulet, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human RBP aa. 1-135

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968619

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-RBP4 (ABIN967847)

Christensen, Moskaug, Vorum, Jacobsen, Gundersen, Nykjaer, Blomhoff, Willnow, Moestrup: "Evidence for an essential role of megalin in transepithelial transport of retinol." dans: Journal of the American Society of Nephrology : JASN, Vol. 10, Issue 4, pp. 685-95, (1999) (PubMed).

Zanotti, Berni, Monaco: "Crystal structure of liganded and unliganded forms of bovine plasma retinol-binding protein." dans: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10728-38, (1993) (PubMed).

Zanotti, Malpeli, Berni: "The interaction of N-ethyl retinamide with plasma retinol-binding protein (RBP) and the crystal structure of the retinoid-RBP complex at 1.9-A resolution." dans: The Journal of biological chemistry, Vol. 268, Issue 33, pp. 24873-9, (1993) (PubMed).

Zanotti, Ottonello, Berni, Monaco: "Crystal structure of the trigonal form of human plasma retinol-binding protein at 2.5 A resolution." dans: Journal of molecular biology, Vol. 230, Issue 2, pp. 613-24, (1993) (PubMed).

Blomhoff, Green, Berg, Norum: "Transport and storage of vitamin A." dans: Science (New York, N.Y.), Vol. 250, Issue 4979, pp. 399-404, (1990) (PubMed).

Détails sur RBP4

Antigène: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Autre désignation: RBP

Sujet: Retinol, a metabolic precursor of retinal and retinoic acid, is transported in plasma bound to a carrier protein known as retinol-binding protein (RBP). RBP is the only retinoid-specific binding protein that has been identified in plasma. The presence of RBP increases the stability of retinol as it is transported from its stores to target cells. In plasma, the 21 kDa RBP forms a tight complex with thyroxine-binding transthyretin (TTR), a homotetramer of about 55 kDa. Association of RBP with TTR increases the stability of the retinol-RBP complex. It is believed that this association prevents filtration of the relatively small RBP through the kidney glomeruli. Once retinol is delivered to its target cells, the resulting retinol-free RBP has a reduced affinity for TTR and thus can be selectively cleared from the circulation by filtration through kidney glomeruli. The three dimensional structures of human and bovine RBP have been compared and appear to be very similar.

Poids moléculaire: 21 kDa

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response