RAB5 anticorps (AA 1-215)

N° du produit ABIN967882

Détail du produit anti-RAB5 anticorps

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Épitope: AA 1-215

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAB5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Rab5 aa. 1-215

Clone: 15-Rab5

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAB5 (ABIN967882)

Huang, Imamura, Olefsky: "Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 23, pp. 13084-9, (2001) (PubMed).

Wang, Hu, Zimmermann, Kilimann: "Rim1 and rabphilin-3 bind Rab3-GTP by composite determinants partially related through N-terminal alpha -helix motifs." dans: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32480-8, (2001) (PubMed).

Pinkoski, Hobman, Heibein, Tomaselli, Li, Seth, Froelich, Bleackley: "Entry and trafficking of granzyme B in target cells during granzyme B-perforin-mediated apoptosis." dans: Blood, Vol. 92, Issue 3, pp. 1044-54, (1998) (PubMed).

Li, Stahl: "Structure-function relationship of the small GTPase rab5." dans: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." dans: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

Détails sur RAB5

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Autre désignation: Rab5 (RAB5A Produits)

Synonymes: anticorps RAB5, anticorps 2410015H04Rik, anticorps AI663973, anticorps AU021172, anticorps nnyRab5a, anticorps DDBDRAFT_0168594, anticorps DDBDRAFT_0229401, anticorps DDB_0168594, anticorps DDB_0229401, anticorps rab5, anticorps RAB5A, anticorps Rab5a, anticorps rab5al, anticorps zgc:56644, anticorps fj38g08, anticorps hm:zehn1144, anticorps rab5a, anticorps wu:fj38g08, anticorps RAB5A, member RAS oncogene family, anticorps rab5A protein, anticorps Rab5a, GTPase, anticorps predicted protein, anticorps Rab GTPase, anticorps RAB5A, member RAS oncogene family L homeolog, anticorps RAB5A, member RAS oncogene family, b, anticorps RAB5A, member RAS oncogene family, a, anticorps RAB5A, anticorps Rab5a, anticorps rab5A, anticorps rab5a, anticorps rab5a.L, anticorps rab5ab, anticorps rab5aa

Sujet: Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation, which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 is involved in regulation of early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and organelle membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 insertion into membranes is a multistep process in which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

Poids moléculaire: 25 kDA

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity