RAN anticorps (AA 7-171)

N° du produit ABIN967913

L’anticorps Souris Monoclonal anti-RAN a été validé pour WB, IHC, IF et IP. Il convient pour détecter RAN dans des échantillons de Humain, Souris, Rat, Chien et Poulet. Il y a 5+ publications disponibles.

Aperçu rapide pour RAN anticorps (AA 7-171) (ABIN967913)

Antigène: RAN (RAN, Member RAS Oncogene Family (RAN))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 20-Ran

Détail du produit anti-RAN anticorps

Épitope: AA 7-171

Réactivité croisée: Poulet, Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ran aa. 7-171

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAN (ABIN967913)

Nemergut, Lindsay, Brownawell, Macara: "Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor." dans: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 17385-8, (2002) (PubMed).

Schwoebel, Ho, Moore: "The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion." dans: The Journal of cell biology, Vol. 157, Issue 6, pp. 963-74, (2002) (PubMed).

Faleiro, Lazebnik: "Caspases disrupt the nuclear-cytoplasmic barrier." dans: The Journal of cell biology, Vol. 151, Issue 5, pp. 951-9, (2000) (PubMed).

Iborra, Jackson, Cook: "The path of RNA through nuclear pores: apparent entry from the sides into specialized pores." dans: Journal of cell science, Vol. 113 Pt 2, pp. 291-302, (2000) (PubMed).

Drivas, Shih, Coutavas, Rush, DEustachio: "Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line." dans: Molecular and cellular biology, Vol. 10, Issue 4, pp. 1793-8, (1990) (PubMed).

Détails sur RAN

Antigène: RAN (RAN, Member RAS Oncogene Family (RAN))

Autre désignation: Ran

Sujet: Ran is a highly conserved GTPase that is ubiquitously expressed. At steady-state, 80-90% of cellular Ran is located in the nucleus, with the remainder in the cytoplasm. The human TC4/Ran cDNA was originally isolated based on the predicted homology of its encoded protein product to Ras. Unlike other members of the Ras superfamily, Ran does not appear to contain signals for lipid modification, nor does it appear to show membrane localization. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 28kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1.

Poids moléculaire: 25 kDa

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus