Flotillin 2 anticorps (AA 122-379)

N° du produit ABIN967932

Cet anticorps anti-Flotillin 2 est un anticorps Souris Monoclonal détectant Flotillin 2 dans WB, IHC, IF et IP. Adapté pour Humain, Rat, Souris, Chien et Poulet. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour Flotillin 2 anticorps (AA 122-379) (ABIN967932)

Antigène: Flotillin 2 (FLOT2)

Reactivité: Humain, Rat, Souris, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Flotillin 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 29-Flotillin

Détail du produit anti-Flotillin 2 anticorps

Épitope: AA 122-379

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human ESA aa. 122-379

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Flotillin 2 (ABIN967932)

Nebl, Pestonjamasp, Leszyk, Crowley, Oh, Luna: "Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes." dans: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43399-409, (2002) (PubMed).

Salzer, Hinterdorfer, Hunger, Borken, Prohaska: "Ca(++)-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin." dans: Blood, Vol. 99, Issue 7, pp. 2569-77, (2002) (PubMed).

Rybin, Xu, Lisanti, Steinberg: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." dans: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, (2001) (PubMed).

Stuermer, Lang, Kirsch, Wiechers, Deininger, Plattner: "Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2." dans: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3031-45, (2001) (PubMed).

Schroeder, Stewart-Galetka, Mandavilli, Parry, Goldsmith, Duvic: "Cloning and characterization of a novel epidermal cell surface antigen (ESA)." dans: The Journal of biological chemistry, Vol. 269, Issue 31, pp. 19983-91, (1994) (PubMed).

Détails sur Flotillin 2

Antigène: Flotillin 2 (FLOT2)

Autre désignation: Flotillin-2

Sujet: Flotillin-2/ESA (epidermal surface antigen) plays a role in intercellular epidermal adhesion. Epidermal surface proteins have an important role in normal epidermal maintenance and function. In addition, they are involved in various autoimmune diseases in which auto-antibodies bind to normal epidermal cell surface proteins. This binding causes cell disadherence that leads to blister formation. Such antibodies have been utilized to identify new proteins involved in epidermal cell adhesion. One of these antibodies, ECS-1, was used to isolate Flotillin-2/ESA. The single copy ESA gene is located on the long arm of human chromosome 17, in the region containing the gene for neurofibromatosis 1 (NF1). ESA mRNA is expressed in cultured keratinocytes, melanocytes, fibroblasts, and carcinoma and melanoma cell lines. Flotillin-2/ESA may play a critical role in epidermal structure and maintenance.
Synonyms: ESA

Poids moléculaire: 42 kDa