EEA1 anticorps (AA 3-281)

N° du produit ABIN967983

Cet anticorps anti-EEA1 est un anticorps Souris Monoclonal détectant EEA1 dans WB, IHC, IP et IF. Adapté pour Humain, Rat, Chien et Poulet. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour EEA1 anticorps (AA 3-281) (ABIN967983)

Antigène: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivité: Humain, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EEA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 14-EEA1

Détail du produit anti-EEA1 anticorps

Épitope: AA 3-281

Réactivité croisée: Rat (Rattus), Poulet, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human EEA1 aa. 3-281

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-EEA1 (ABIN967983)

van Dam, Stoorvogel: "Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles." dans: Molecular biology of the cell, Vol. 13, Issue 1, pp. 169-82, (2002) (PubMed).

Yan, Han, Miao, Greengard, Xu: "The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate." dans: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36788-96, (2001) (PubMed).

Allan, Moyer, Balch: "Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion." dans: Science (New York, N.Y.), Vol. 289, Issue 5478, pp. 444-8, (2000) (PubMed).

Mullock, Smith, Ihrke, Bright, Lindsay, Parkinson, Brooks, Parton, James, Luzio, Piper: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." dans: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3137-53, (2000) (PubMed).

Mu, Callaghan, Steele-Mortimer, Stenmark, Parton, Campbell, McCluskey, Yeo, Tock, Toh: "EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif." dans: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13503-11, (1995) (PubMed).

Détails sur EEA1

Antigène: EEA1 (Early Endosome Antigen 1 (EEA1))

Autre désignation: EEA1

Sujet: Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly alpha-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine finger motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 180 kDa

Pathways: Toll-Like Receptors Cascades