Ezrin anticorps (AA 391-515)

N° du produit ABIN968057

L’anticorps Souris Monoclonal anti-Ezrin a été validé pour WB, IHC, IP et IF. Il convient pour détecter Ezrin dans des échantillons de Humain et Chien. Il y a 5+ publications disponibles.

Aperçu rapide pour Ezrin anticorps (AA 391-515) (ABIN968057)

Antigène: Ezrin (EZR)

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ezrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 18-Ezrin

Détail du produit anti-Ezrin anticorps

Épitope: AA 391-515

Réactivité croisée: Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ezrin aa. 391-515

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968619, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Ezrin (ABIN968057)

Perez, Kinoshita, Hitoshi, Payan, Kitamura, Nolan, Lorens: "Activation of the PKB/AKT pathway by ICAM-2." dans: Immunity, Vol. 16, Issue 1, pp. 51-65, (2002) (PubMed).

Anastasiadis, Moon, Thoreson, Mariner, Crawford, Zheng, Reynolds: "Inhibition of RhoA by p120 catenin." dans: Nature cell biology, Vol. 2, Issue 9, pp. 637-44, (2001) (PubMed).

Defacque, Egeberg, Habermann, Diakonova, Roy, Mangeat, Voelter, Marriott, Pfannstiel, Faulstich, Griffiths: "Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes." dans: The EMBO journal, Vol. 19, Issue 2, pp. 199-212, (2000) (PubMed).

Mohler, Kreda, Boucher, Sudol, Stutts, Milgram: "Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50." dans: The Journal of cell biology, Vol. 147, Issue 4, pp. 879-90, (1999) (PubMed).

Berryman, Gary, Bretscher: "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." dans: The Journal of cell biology, Vol. 131, Issue 5, pp. 1231-42, (1996) (PubMed).

Détails sur Ezrin

Antigène: Ezrin (EZR)

Autre désignation: Ezrin

Sujet: First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.

Poids moléculaire: 80 kDa

Pathways: Maintenance of Protein Location