ITGA5 anticorps (AA 853-1002)

N° du produit ABIN968077

Détail du produit anti-ITGA5 anticorps

Antigène: ITGA5 (Integrin, alpha 5 (ITGA5))

Épitope: AA 853-1002

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Integrin alpha5 aa. 853-1002

Clone: 1-CD49e

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ITGA5 (ABIN968077)

Sottile, Hocking: "Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions." dans: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3546-59, (2002) (PubMed).

Alahari, Lee, Juliano: "Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration." dans: The Journal of cell biology, Vol. 151, Issue 6, pp. 1141-54, (2001) (PubMed).

Aplin, Short, Juliano: "Anchorage-dependent regulation of the mitogen-activated protein kinase cascade by growth factors is supported by a variety of integrin alpha chains." dans: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31223-8, (1999) (PubMed).

Zheng, Fang, Hakomori et al.: "Functional role of N-glycosylation in alpha 5 beta 1 integrin receptor. De-N-glycosylation induces dissociation or altered association of alpha 5 and beta 1 subunits and concomitant loss of ..." dans: The Journal of biological chemistry, Vol. 269, Issue 16, pp. 12325-31, (1994) (PubMed).

Bauer, Varner, Schreiner, Kornberg, Nicholas, Juliano: "Functional role of the cytoplasmic domain of the integrin alpha 5 subunit." dans: The Journal of cell biology, Vol. 122, Issue 1, pp. 209-21, (1993) (PubMed).

Détails sur ITGA5

Antigène: ITGA5 (Integrin, alpha 5 (ITGA5))

Autre désignation: CD49e (ITGA5 Produits)

Synonymes: anticorps ITGA5, anticorps Cd49e, anticorps Fnra, anticorps VLA5, anticorps CD49e, anticorps FNRA, anticorps VLA5A, anticorps Itga5_retired, anticorps integrin subunit alpha 5, anticorps integrin alpha 5 (fibronectin receptor alpha), anticorps ITGA5, anticorps Itga5

Sujet: Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. They are transmembrane heterodimers composed of alpha and beta subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 alpha and 9 beta subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The integrin alpha5 subunit is 1049 amino acids with a molecular weight of 150-165 kDa. alpha5 and beta1 form a functional fibronectin receptor which is important for vascular development in mice. Both subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin alpha5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alpha5

Poids moléculaire: 150 kDa

Pathways: Integrin Complex