ALOX5 anticorps (AA 442-590)

N° du produit ABIN968116

Détail du produit anti-ALOX5 anticorps

Antigène: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Épitope: AA 442-590

Reactivité: Humain, Rat, Souris, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ALOX5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Réactivité croisée: Poulet, Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human 5-Lipoxygenase aa. 442-590

Clone: 33-5

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968554

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-ALOX5 (ABIN968116)

Hundley, Prasad, Beaven: "Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1629-36, (2001) (PubMed).

Zaitsu, Hamasaki, Matsuo, Kukita, Tsuji, Miyazaki, Hayasaki, Muro, Yamamoto, Kobayashi, Ichimaru, Kohashi, Miyazaki: "New induction of leukotriene A(4) hydrolase by interleukin-4 and interleukin-13 in human polymorphonuclear leukocytes." dans: Blood, Vol. 96, Issue 2, pp. 601-9, (2000) (PubMed).

Lepley, Muskardin, Fitzpatrick: "Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase." dans: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6179-84, (1996) (PubMed).

Lepley, Fitzpatrick: "5-Lipoxygenase contains a functional Src homology 3-binding motif that interacts with the Src homology 3 domain of Grb2 and cytoskeletal proteins." dans: The Journal of biological chemistry, Vol. 269, Issue 39, pp. 24163-8, (1994) (PubMed).

Matsumoto, Funk, Rådmark, Höög, Jörnvall, Samuelsson: "Molecular cloning and amino acid sequence of human 5-lipoxygenase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 1, pp. 26-30, (1988) (PubMed).

Détails sur ALOX5

Antigène: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Autre désignation: 5-Lipoxygenase (ALOX5 Produits)

Synonymes: anticorps 5-LO, anticorps 5-LOX, anticorps 5LPG, anticorps LOG5, anticorps 5LO, anticorps 5LX, anticorps AI850497, anticorps F730011J02, anticorps LOX5A, anticorps si:dkey-194n13.2, anticorps lox5, anticorps arachidonate 5-lipoxygenase, anticorps arachidonate 5-lipoxygenase a, anticorps Arachidonate 5-lipoxygenase, anticorps ALOX5, anticorps Alox5, anticorps alox5a, anticorps AFUA_4G02770, anticorps AOR_1_1362014, anticorps lox5

Sujet: 5-Lipoxygenase (5-LO) is the initial enzyme that converts arachidonic acid to leukotrienes, which are important inflammatory and vasoconstrictive metabolites. It is activated in response to a number of stimuli, such as differentiation and allergen challenges. The 5-LO gene, abundantly expressed in placenta, lung, and leukocytes, encodes a protein of 674 amino acids with an apparent molecular weight of 78kDa. 5-LO is a Ca2+ and ATP-dependent enzyme that translocates from the cytosol to either a nuclear or plasma membrane compartment following activation. A proline-rich domain of 5-LO (amino acids 566-577) has been identified as a binding site for the PTyr-binding protein, Grb2. This Grb2 site links tyrosine kinases with activation and redistribution of 5-LO. Furthermore, tyrosine kinase inhibitors increase the activity of 5-LO and block the enzyme's subcellular redistribution.

Poids moléculaire: 79 kDa