AKAP1 anticorps (AA 66-212)

N° du produit ABIN968136

Cet anticorps anti-AKAP1 est un anticorps Souris Monoclonal détectant AKAP1 dans WB, IHC, IF et IP. Adapté pour Humain et Rat. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour AKAP1 anticorps (AA 66-212) (ABIN968136)

Antigène: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 6-AKAP149

Détail du produit anti-AKAP1 anticorps

Épitope: AA 66-212

Réactivité croisée: Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human AKAP149 aa. 66-212

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-AKAP1 (ABIN968136)

Schillace, Andrews, Liberty, Davey, Carr: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, (2002) (PubMed).

Tanji, Yamamoto, Yorioka, Kohno, Kikuchi, Kikuchi: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." dans: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, (2002) (PubMed).

Steen, Collas: "Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression." dans: The Journal of cell biology, Vol. 153, Issue 3, pp. 621-6, (2001) (PubMed).

DellAcqua, Scott: "Protein kinase A anchoring." dans: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 12881-4, (1997) (PubMed).

Trendelenburg, Hummel, Riecken, Hanski: "Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain." dans: Biochemical and biophysical research communications, Vol. 225, Issue 1, pp. 313-9, (1996) (PubMed).

Détails sur AKAP1

Antigène: AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))

Autre désignation: AKAP149

Sujet: The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 149 kDa

Pathways: Synaptic Membrane