Afadin anticorps (AA 1091-1233)

N° du produit ABIN968143

Cet anticorps Souris Monoclonal détecte spécifiquement Afadin dans WB, IHC, IP et IF. Il présente une réactivité envers Humain, Rat et Souris et a été mentionné dans 5+ publications.

Aperçu rapide pour Afadin anticorps (AA 1091-1233) (ABIN968143)

Antigène: Afadin (MLLT4)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Afadin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 35-AF6

Détail du produit anti-Afadin anticorps

Épitope: AA 1091-1233

Réactivité croisée: Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human AF6 aa. 1091-1233

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Afadin (ABIN968143)

Cordenonsi, DAtri, Hammar, Parry, Kendrick-Jones, Shore, Citi: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." dans: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, (2000) (PubMed).

Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." dans: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, (2000) (PubMed).

Buchert, Schneider, Meskenaite, Adams, Canaani, Baechi, Moelling, Hovens: "The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinases at specialized sites of cell-cell contact in the brain." dans: The Journal of cell biology, Vol. 144, Issue 2, pp. 361-71, (1999) (PubMed).

Kuriyama, Harada, Kuroda, Yamamoto, Nakafuku, Iwamatsu, Yamamoto, Prasad, Croce, Canaani, Kaibuchi: "Identification of AF-6 and canoe as putative targets for Ras." dans: The Journal of biological chemistry, Vol. 271, Issue 2, pp. 607-10, (1996) (PubMed).

Prasad, Gu, Alder, Nakamura, Canaani, Saito, Huebner, Gale, Nowell, Kuriyama: "Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation." dans: Cancer research, Vol. 53, Issue 23, pp. 5624-8, (1994) (PubMed).

Détails sur Afadin

Antigène: Afadin (MLLT4)

Autre désignation: AF6

Sujet: AF6 (ALL-1 Fusion partner in chromosome 6) was discovered as the fusion partner of ALL-1. These genes undergo translocation and fusion in acute myeloid leukemias. AF6, or p180, was also identified as a Ras-interacting protein isolated from bovine brain. AF6 binds to the GTP-Ras but not to the inactive GDP, or ineffective forms of Ras. The interaction between AF6 and Ras may regulate the Ras signaling pathway since this association was prevented by the binding of c-Raf-1 to Ras. The Ras-binding site is located within amino acids 36-206 of AF6. AF6 is 1612 amino acids in length with homology to the DLG family of proteins located in cell-cell junctions, suggesting AF6 might be important in cell-to-cell communicative events. Furthermore, AF6 has the GLGF motif characteristic of DLG proteins that include Drosophilia's Canoe, implicated in the developmental pathway triggered by Notch activation. Like AF6, Canoe was also identified as a Ras-binding protein. The amino acid sequence and biochemical property similarities between Canoe and AF6 indicate that the latter may be important in developmental processes regulated by Ras.
Synonyms: ALL-1 Fusion partner in chromosome 6

Poids moléculaire: 182 kDa

Pathways: Cell-Cell Junction Organization