ARF3 anticorps (AA 1-181)

N° du produit ABIN968175

Cet anticorps anti-ARF3 est un anticorps Souris Monoclonal détectant ARF3 dans WB, IHC, IF et IP. Adapté pour Humain, Souris, Rat, Poulet, Chien et Grenouille. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour ARF3 anticorps (AA 1-181) (ABIN968175)

Antigène: ARF3 (ADP-Ribosylation Factor 3 (ARF3))

Reactivité: Humain, Souris, Rat, Poulet, Chien, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ARF3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 41-ARF3

Détail du produit anti-ARF3 anticorps

Épitope: AA 1-181

Réactivité croisée: Rat (Rattus), Souris, Poulet, Chien, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human ARF3 aa. 1-181

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ARF3 (ABIN968175)

Lee, Stevens, Hsu, Tsai, Lee, Moss, Vaughan: "Expression in human endothelial cells of ADP-ribosylation factors, 20-kDa guanine nucleotide-binding proteins involved in the initiation of vesicular transport." dans: Journal of molecular and cellular cardiology, Vol. 28, Issue 9, pp. 1911-20, (1997) (PubMed).

Morinaga, Tsai, Moss, Vaughan: "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12856-60, (1996) (PubMed).

Cockcroft, Thomas, Fensome, Geny, Cunningham, Gout, Hiles, Totty, Truong, Hsuan: "Phospholipase D: a downstream effector of ARF in granulocytes." dans: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 523-6, (1994) (PubMed).

Moss, Vaughan: "ADP-ribosylation factors, 20,000 M(r) guanine nucleotide-binding protein activators of cholera toxin and components of intracellular vesicular transport systems." dans: Cellular signalling, Vol. 5, Issue 4, pp. 367-79, (1993) (PubMed).

Kahn, Radding: "Separation of the presynaptic and synaptic phases of homologous pairing promoted by recA protein." dans: The Journal of biological chemistry, Vol. 259, Issue 12, pp. 7495-503, (1984) (PubMed).

Détails sur ARF3

Antigène: ARF3 (ADP-Ribosylation Factor 3 (ARF3))

Autre désignation: ARF3

Sujet: The ADP-ribosylation factors (ARFs) belong to the multigene family of small GTPases capable of activating cholera toxin. ARFs fall into three different classes: Class I is composed of ARF-1, ARF-2, and ARF-3, Class II consists of ARF-4 and ARF-5, and Class III includes ARF6. Unique to ARFs is their lack of intrinsic GTP hydrolysis activity, a high affinity for GDP in a Mg2+-dependent manner, and phospholipid requirement for nucleotide exchange. ARFs are involved in intravesicular acidification of microsomal vesicles, endosome fusion, nuclear membrane assembly, and formation of clathrin-coated vesicles. In addition, GTP and ARF-3 are required for the activation of phospholipase D (PLD), an early cellular response triggered by the binding of receptors on the cell surface in response to numerous extracellular signals. Although predominantly cytosolic, ARF-3 can be translocated to cellular membranes upon cellular stimulation. The transition between the GDP-bound ARF-3 to the GTP-ARF-3 is facilitated by a high molecular weight guanine nucleotide-exchange factor sensitive to brefeldin. ARF-3 has been reported to be the most abundant in brain, kidney, and liver.
Synonyms: ADP Ribosylation Factor-3

Poids moléculaire: 20 kDa

Pathways: Inositol Metabolic Process, Cellular Glucan Metabolic Process