DIAPH1 anticorps (AA 41-153)

N° du produit ABIN968212

Cet anticorps Souris Monoclonal détecte spécifiquement DIAPH1 dans WB, IHC, IF et IP. Il présente une réactivité envers Humain, Souris, Rat et Chien et a été mentionné dans 3+ publications.

Aperçu rapide pour DIAPH1 anticorps (AA 41-153) (ABIN968212)

Antigène: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DIAPH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 51-mDia1

Détail du produit anti-DIAPH1 anticorps

Épitope: AA 41-153

Réactivité croisée: Rat (Rattus), Humain, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse p140mDia aa. 41-153

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-DIAPH1 (ABIN968212)

Li, Higgs: "The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition." dans: Current biology : CB, Vol. 13, Issue 15, pp. 1335-40, (2003) (PubMed).

Pennisi: "The architecture of hearing." dans: Science (New York, N.Y.), Vol. 278, Issue 5341, pp. 1223-4, (1997) (PubMed).

Watanabe, Madaule, Reid, Ishizaki, Watanabe, Kakizuka, Saito, Nakao, Jockusch, Narumiya: "p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin." dans: The EMBO journal, Vol. 16, Issue 11, pp. 3044-56, (1997) (PubMed).

Détails sur DIAPH1

Antigène: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Autre désignation: p140mDia

Sujet: GTPases like Ras, Rho, cdc42Hs, and Rac modulate a multitude of cellular functions like cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis. mDia1, also known as p140mDia and Drf1, is the mammalian homologue of Drosophila's diaphanous, a protein essential for cytokinesis. The 1255 amino acid mDia1 is widely expressed, with a Rho binding domain at the NH2-terminal region, a central polyproline region, and an FH2 domain. This protein also shares homology with the yeast Bn1p essential for budding and the mouse formin necessary for proper limb development. mDia1 binds to GTP-Rho and to the actin-binding protein profilin-all three proteins are co-localized at the lamellipodia in cultured cells. The overexpression of mDia1 promoted the formation of actin filaments, implicating this protein in cell motility events regulated by Rho. mDia1 was also found consistently mutated in familial deafness.
Synonyms: p140mDia, Drf1

Poids moléculaire: 140 kDa

Pathways: Sensory Perception of Sound