UBE2L3 anticorps (AA 15-133)

N° du produit ABIN968215

Cet anticorps Souris Monoclonal détecte spécifiquement UBE2L3 dans WB, IHC, IF et IP. Il présente une réactivité envers Humain, Souris, Rat, Chien et Grenouille et a été mentionné dans 2+ publications.

Aperçu rapide pour UBE2L3 anticorps (AA 15-133) (ABIN968215)

Antigène: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivité: Humain, Souris, Rat, Chien, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UBE2L3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 20-UbcH7

Détail du produit anti-UBE2L3 anticorps

Épitope: AA 15-133

Réactivité croisée: Souris, Rat (Rattus), Chien, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human UbcH7 aa. 15-133

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-UBE2L3 (ABIN968215)

Hagglund, Van Sant, Lopez, Roizman: "Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 631-6, (2002) (PubMed).

Shimura, Schlossmacher, Hattori, Frosch, Trockenbacher, Schneider, Mizuno, Kosik, Selkoe: "Ubiquitination of a new form of alpha-synuclein by parkin from human brain: implications for Parkinson's disease." dans: Science (New York, N.Y.), Vol. 293, Issue 5528, pp. 263-9, (2001) (PubMed).

Détails sur UBE2L3

Antigène: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Autre désignation: UbcH7

Sujet: Progression of the mammalian cell cycle is regulated mainly by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome-a cytoplasmic complex of proteases. Several human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been identified and found involved in critical processes such as cell cycle progression and apoptosis. UbcH7 was characterized as an E6-AP-interacting protein required for the ubiquitination of p53. E6-AP is the ubiquitin ligase used as bait in the search for interacting proteins required for the proper ubiquitination of cellular targets. UbcH7 interacts with the C-terminal region of E6-AP named the hect domain. Therefore, UbcH7 is another protein, like Ubc9, involved in the degradation and regulation of critical proteins in the cell cycle. This antibody is routinely tested by western blot analysis.
Synonyms: Ubiquitin Conjugating Enzyme H7

Poids moléculaire: 17-18 kDa