CHRNA1 anticorps (AA 332-457)

N° du produit ABIN968298

Détail du produit anti-CHRNA1 anticorps

Antigène: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Épitope: AA 332-457

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHRNA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Acetylcholine Receptor alpha aa. 332-457

Clone: 26-Acetylcholine Receptor alpha

Isotype: IgG2a

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CHRNA1 (ABIN968298)

Kincer, Uittenbogaard, Dressman, Guerin, Febbraio, Guo, Smart: "Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction." dans: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23525-33, (2002) (PubMed).

Spencer, Guyon, Sorimachi, Potts, Richard, Herasse, Chamberlain, Dalkilic, Kunkel, Beckmann: "Stable expression of calpain 3 from a muscle transgene in vivo: immature muscle in transgenic mice suggests a role for calpain 3 in muscle maturation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 13, pp. 8874-9, (2002) (PubMed).

Garchon, Djabiri, Viard, Gajdos, Bach: "Involvement of human muscle acetylcholine receptor alpha-subunit gene (CHRNA) in susceptibility to myasthenia gravis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 11, pp. 4668-72, (1994) (PubMed).

Schröder, Reinhardt-Maelicke, Schrattenholz, McLane, Kretschmer, Conti-Tronconi, Maelicke: "Monoclonal antibodies FK1 and WF6 define two neighboring ligand binding sites on Torpedo acetylcholine receptor alpha-polypeptide." dans: The Journal of biological chemistry, Vol. 269, Issue 14, pp. 10407-16, (1994) (PubMed).

Tsim, Greenberg, Rimer, Randall, Salpeter: "Transcripts for the acetylcholine receptor and acetylcholine esterase show distribution differences in cultured chick muscle cells." dans: The Journal of cell biology, Vol. 118, Issue 5, pp. 1201-12, (1992) (PubMed).

Détails sur CHRNA1

Antigène: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Autre désignation: Acetylcholine Receptor alpha (CHRNA1 Produits)

Synonymes: anticorps CHRNA1, anticorps ACHRA, anticorps ACHRD, anticorps CHRNA, anticorps CMS2A, anticorps FCCMS, anticorps SCCMS, anticorps NACHRA1, anticorps AI385656, anticorps AI608266, anticorps Achr-1, anticorps Acra, anticorps cholinergic receptor nicotinic alpha 1 subunit, anticorps cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle), anticorps CHRNA1, anticorps Chrna1

Sujet: Acetylcholine is an amine neurotransmitter at the neuromuscular junction. It is released from the presynaptic membrane of a cholinergic synapse into the synaptic cleft. It diffuses across the cleft and binds acetylcholine receptors (AChR) on the postsynaptic membrane. Receptor binding induces postsynaptic membrane depolarization and the generation of an action potential that produces effects such as muscle contraction. The AChR is a 250kDa pentameric complex of four transmembrane subunits in a stoichiometry of alpha2betagammadelta. In response to ligand binding, all subunits participate in the formation of an integral cation channel. However, the acetylcholine binding site is primarily within the alpha subunit. Myasthenia gravis (MG) is an autoimmune condition in which AchR levels are decreased. Autoantibodies bind and crosslink the AchRs leading to their internalization and degradation. This results in a decreased number of functional AChRs. Patients develop muscular weakness and some voluntary muscle fatigue. However, development of MG is also affected by genetic factors. One of the allelic forms of the AChRalpha gene appears to significantly contribute to MG susceptibility.

Poids moléculaire: 49 kDa

Pathways: Skeletal Muscle Fiber Development