SRPK1 anticorps (AA 312-434)

N° du produit ABIN968353

Cet anticorps Souris Monoclonal détecte spécifiquement SRPK1 dans WB et IF. Il présente une réactivité envers Humain, Souris, Rat et Chien et a été mentionné dans 2+ publications.

Aperçu rapide pour SRPK1 anticorps (AA 312-434) (ABIN968353)

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SRPK1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 12-SRPK1

Détail du produit anti-SRPK1 anticorps

Épitope: AA 312-434

Réactivité croisée: Chien, Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human SRPK1 aa.312-434

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SRPK1 (ABIN968353)

Wang, Lin, Dyck, Yeakley, Songyang, Cantley, Fu: "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells." dans: The Journal of cell biology, Vol. 140, Issue 4, pp. 737-50, (1998) (PubMed).

Colwill, Feng, Yeakley, Gish, Cáceres, Pawson, Fu: "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors." dans: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24569-75, (1996) (PubMed).

Détails sur SRPK1

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Autre désignation: SRPK1

Sujet: Mammalian cell pre-mRNA splicing is mediated by the spliceosome, a multi-component complex that contains two types of splicing factors: small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP factors. Interactions between snRNPs and pre-mRNA ensures proper establishment of a catalytic core for the splicing reaction. However, these interactions are mediated by the non-snRNP factors. The super family of Arg/Ser-rich (RS) domain containing splicing factors are well known non-snRNPs. All of these proteins share a similar structure consisting of an N-terminal RNA recognition motif and a C-terminal RS domain. However, different SR factors have distinct specificities and function is regulated by their level of expression and by reversible phosphorylation. Two families of kinases phosphorylate SR domain-containing proteins: SR protein-specific kinases (SRPK1 and 2) and Clk/Sty. SRPL1 is specific for SR domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, domain-containing splicing factors because it recognizes only Arg and phosphorylates only Ser. SRPK1 is expressed predominately in the pancreas, whereas SRPK2 is highly expressed in brain. SRPKs affect splice-site selection and are thought to affect alternative splicing.

Poids moléculaire: 92 kDa

Pathways: Toll-Like Receptors Cascades