NCOA3 anticorps (AA 376-389)

N° du produit ABIN968368

Cet anticorps Souris Monoclonal détecte spécifiquement NCOA3 dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 5+ publications.

Aperçu rapide pour NCOA3 anticorps (AA 376-389) (ABIN968368)

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NCOA3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 34-AIB

Détail du produit anti-NCOA3 anticorps

Épitope: AA 376-389

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human AIB-1 aa. 376-389

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NCOA3 (ABIN968368)

Louie, Yang, Ma, Xu, Zou, Kung, Chen: "Androgen-induced recruitment of RNA polymerase II to a nuclear receptor-p160 coactivator complex." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2226-30, (2003) (PubMed).

Lauritsen, List, Reiter, Wellstein, Riegel: "A role for TGF-beta in estrogen and retinoid mediated regulation of the nuclear receptor coactivator AIB1 in MCF-7 breast cancer cells." dans: Oncogene, Vol. 21, Issue 47, pp. 7147-55, (2002) (PubMed).

Reiter, Wellstein, Riegel: "An isoform of the coactivator AIB1 that increases hormone and growth factor sensitivity is overexpressed in breast cancer." dans: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39736-41, (2001) (PubMed).

Eng, Barsalou, Akutsu, Mercier, Zechel, Mader, White: "Different classes of coactivators recognize distinct but overlapping binding sites on the estrogen receptor ligand binding domain." dans: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 28371-7, (1998) (PubMed).

Anzick, Kononen, Walker, Azorsa, Tanner, Guan, Sauter, Kallioniemi, Trent, Meltzer: "AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer." dans: Science (New York, N.Y.), Vol. 277, Issue 5328, pp. 965-8, (1997) (PubMed).

Détails sur NCOA3

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Autre désignation: AIB-1

Sujet: Signal transduction via nuclear hormone receptors is important for cell growth and differentiation, development, and homeostasis. Nuclear hormone receptors are ligand-activated transcription factors that modulate target gene expression. These ligand/receptor complexes also interact with transcriptional coactivators which enhance ligand-dependent transcription. Various classes of coactivators have been identified, including SRC-1 and its related proteins, such as TIF-2/GRIP-1, RIP140, AIB-1, and TIF-1alpha and -1beta. AIB-1 (Amplified In Breast cancer-1) is also known as pCIP, RAC3, and TRAM-1. It interacts with estrogen receptor (ER) and is overexpressed in breast cancer biopsies and several breast and ovarian cancer cell lines. Similar to SRC-1 and TIF2, AIB-1 contains a basic helix-loop-helix (bHLH) domain followed by a PAS (Per/Arnt/Sim) region, serine and threonine rich regions, and a charged cluster. In addition, AIB-1 contains three copies of the conserved LXXLL motif which is critical to its interaction with the nuclear receptor. Thus, AIB-1 is a coactivator of nuclear receptors that may participate in the development of steroid-dependent cancers.
Synonyms: Amplified In Breast cancer-1, pCIP, RAC3, TRAM-1

Poids moléculaire: 160 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha