CG7626 Gene Product From Transcript CG7626-RA (SPT5) (AA 866-985) anticorps

N° du produit ABIN968370

Cité dans 1 publication. Cet anticorps anti-CG7626 Gene Product From Transcript CG7626-RA Monoclonal Souris (Clone 17-DSIF) (ABIN968370) détecte spécifiquement CG7626 Gene Product From Transcript CG7626-RA dans IF et WB. L’anticorps est réactif avec des échantillons de Chien, Humain, Souris et Rat.

Aperçu rapide pour CG7626 Gene Product From Transcript CG7626-RA (SPT5) (AA 866-985) anticorps (ABIN968370)

Antigène: CG7626 Gene Product From Transcript CG7626-RA (SPT5)

Reactivité: Chien, Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Clone: 17-DSIF

Détail du produit

Épitope: AA 866-985

Réactivité croisée: Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human DSIF aa. 866-985

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Référence

Kim, Bourgeois, Isel, Churcher, Karn: "Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation." dans: Molecular and cellular biology, Vol. 22, Issue 13, pp. 4622-37, (2002) (PubMed).

Détail du antigène

Antigène: CG7626 Gene Product From Transcript CG7626-RA (SPT5)

Autre désignation: DSIF

Sujet: In living systems, the relative amounts of any protein are controlled at many levels. For example, amounts are affected by protein degradation, regulation of the translational rates of polypeptide synthesis (translational regulation), and control of the rates of mRNA synthesis (transcriptional regulation). Transcriptional regulation involves modulation of the rate-limiting enzyme RNA polymerase. DSIF (DRB sensitivity-inducing factor) is a heterodimeric transcription elongation protein. It is composed of a large subunit of 160 kDa and a small subunit of 14 kDa. These large and the small subunits are homologs of the yeast gene products Stp5 and Stp4, respectively. Spt4 and 5 are transcription factors which are critically important for the activity of RNA polymerase. In conjunction with DRB, DSIF attenuates RNA polymerase II elongation steps. However, in limiting amounts of ribonucleotides, DSIF, by itself, stimulates the elongation rate of RNA polymerase II. Thus, the identification of a human regulator for transcriptional elongation will greatly enhance our understanding of this critical step in mammalian gene expression. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 160 kDa