BCAT1 anticorps (AA 213-335)

N° du produit ABIN968446

Cet anticorps Souris Monoclonal détecte spécifiquement BCAT1 dans WB et IF. Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour BCAT1 anticorps (AA 213-335) (ABIN968446)

Antigène: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BCAT1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 51-ECA39

Détail du produit anti-BCAT1 anticorps

Épitope: AA 213-335

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human ECA39 aa. 213-335

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968535

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-BCAT1 (ABIN968446)

Ben-Yosef, Yanuka, Halle, Benvenisty: "Involvement of Myc targets in c-myc and N-myc induced human tumors." dans: Oncogene, Vol. 17, Issue 2, pp. 165-71, (1998) (PubMed).

Ben-Yosef, Yanuka, Benvenisty: "ECA39 is regulated by c-Myc in human and by a Jun/Fos homolog, Gcn4, in yeast." dans: Oncogene, Vol. 13, Issue 9, pp. 1859-66, (1997) (PubMed).

Eden, Simchen, Benvenisty: "Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases." dans: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20242-5, (1996) (PubMed).

Schuldiner, Eden, Ben-Yosef, Yanuka, Simchen, Benvenisty: "ECA39, a conserved gene regulated by c-Myc in mice, is involved in G1/S cell cycle regulation in yeast." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 14, pp. 7143-8, (1996) (PubMed).

Détails sur BCAT1

Antigène: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Autre désignation: ECA39

Sujet: The c-myc oncogene is important in proliferation, differentiation, apoptosis, and malignant transformation. Products of myc oncogenes are transcription factors that bind to the DNA sequence, CACGTG, and regulate the expression of multiple target genes. Several myc target genes include ECA39, p53, ornithine decarboxylase (ODC), alpha-prothymosin, and Cdc25A. There is a high degree of identity among the mouse, human, and yeast ECA39 proteins. The myc recognition binding site of ECA39 is located 3' to the start site of transcription in the mouse and human genes, but this element is absent in the nematode and yeast. Additionally, the tissue specific expression of ECA39 during embryogenesis is similar between human and mouse. Disruption of the ECA39 gene in yeast results in an increased growth rate in comparison to wild type, such that G1 is shorter. Furthermore, ECA39 is expressed in c-myc induced tumors and displays significant homology to the prokaryotic branched-chain amino acid aminotransferase (BCAT). Thus, ECA39 may be involved in the regulation of the cell cycle, possibly at the G1 to S phase transition.

Poids moléculaire: 43 kDa