NPAT anticorps (AA 681-803)

N° du produit ABIN968484

Cet anticorps anti-NPAT est un anticorps Souris Monoclonal détectant NPAT dans WB et IF. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour NPAT anticorps (AA 681-803) (ABIN968484)

Antigène: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NPAT est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 27-NPAT

Détail du produit anti-NPAT anticorps

Épitope: AA 681-803

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human NPAT aa. 681-803

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NPAT (ABIN968484)

Zhao, Dynlacht, Imai, Hori, Harlow: "Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry." dans: Genes & development, Vol. 12, Issue 4, pp. 456-61, (1998) (PubMed).

Détails sur NPAT

Antigène: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Autre désignation: NPAT

Sujet: Cyclins regulate transitions between cell cycle phases by acting as regulatory subunits of the cyclin-dependent kinases (cdk). The temporal expression of cyclins is tightly regulated and plays a critical role in controlling the enzymatic activity of the cdks. Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 is expressed earlier in the cell cycle than cdc2 and forms complexes with cyclins A, E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important, as is Cyclin A-Cdk2, for the progression from G1 to S phase. One substrate for cyclin E-Cdk2 is a Nuclear Protein mapped to the Ataxia Telangiectasia locus, NPAT. This protein associates with cyclin E-Cdk2 and can be phosphorylated by Cdk2. NPAT protein levels peak at the G1/S boundary and overexpression of NPAT accelerates S phase entry, especially after coexpression of cyclin E-Cdk2. Thus, NPAT is a substrate of cyclin E-Cdk2 that may mediate G1 to S phase transition.
Synonyms: Nuclear Protein Ataxia Telangiectasia

Poids moléculaire: 210 kDa