CYBB anticorps (AA 450-556)

N° du produit ABIN968524

Détail du produit anti-CYBB anticorps

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Épitope: AA 450-556

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CYBB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse gp91[phox] aa. 450-556

Clone: 53-gp91[phox]

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968555, ABIN967389, ABIN967918, ABIN968245

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-CYBB (ABIN968524)

Jacobsen, Skalnik: "YY1 binds five cis-elements and trans-activates the myeloid cell-restricted gp91(phox) promoter." dans: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 29984-93, (1999) (PubMed).

Suzuki, Kumatori, Haagen, Fujii, Sadat, Jun, Tsuji, Roos, Nakamura: "PU.1 as an essential activator for the expression of gp91(phox) gene in human peripheral neutrophils, monocytes, and B lymphocytes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 11, pp. 6085-90, (1998) (PubMed).

Yu, Quinn, Cross, Dinauer: "Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 7993-8, (1998) (PubMed).

Zhen, Yu, Dinauer: "Probing the role of the carboxyl terminus of the gp91phox subunit of neutrophil flavocytochrome b558 using site-directed mutagenesis." dans: The Journal of biological chemistry, Vol. 273, Issue 11, pp. 6575-81, (1998) (PubMed).

Détails sur CYBB

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Autre désignation: gp91 phox (CYBB Produits)

Synonymes: anticorps zgc:64144, anticorps cgd, anticorps gp91-1, anticorps gp91-phox, anticorps gp91phox, anticorps nox2, anticorps NOX2, anticorps cybb, anticorps NOX1, anticorps AMCBX2, anticorps CGD, anticorps GP91-1, anticorps GP91-PHOX, anticorps GP91PHOX, anticorps p91-PHOX, anticorps Nox2, anticorps C88302, anticorps CGD91-phox, anticorps Cgd, anticorps Cyd, anticorps Gp91-phox, anticorps CYBB, anticorps cytochrome b-245, beta polypeptide (chronic granulomatous disease), anticorps cytochrome b-245, beta polypeptide, anticorps cytochrome b-245 heavy chain, anticorps cytochrome b-245, beta polypeptide L homeolog, anticorps cytochrome b-245 beta polypeptide, anticorps cytochrome b-245 beta chain, anticorps NADPH oxidase heavy chain subunit, anticorps cybb, anticorps CYBB, anticorps cybb.L, anticorps Cybb, anticorps GP91-PHOX

Sujet: Although dormant in resting granulocytes, macrophages, and B lymphocytes, the neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. It is an integral membrane cytochrome, b558, which consists of two subunits, gp91[phox] and p21[phox]. Upon stimulation, cytochrome b558 forms a complex with cytosolic proteins, p67[phox], p47[phox], p40[phox], and rac2 and produces superoxide anions in a NADPH-dependent manner. In chronic granulomatous disease (CGD), severe recurrent bacterial and fungal infections result from defective NADPH-oxidase activity. The majority of CGD cases are caused by a defective gp91[phox] gene. gp91[phox], implicated as a docking site for p47[phox], is membrane glycoprotein with multiple N-terminal hydrophobic domains and a hydrophilic C-terminus. The expression of gp91[phox] is restricted to terminally differentiated phagocytes and B lymphocytes. This cell type- and developmental stage-specific expression may be controlled by transcriptional activators, such as PU.1 and YY1, and transcriptional repressors, such as CCAAT displacement protein (CDP). Endogenous mouse gp91phox shows 58 kDa band, instead of 91 kDa observed in human. Both mouse and human deglycosylated gp91 are 54 kDa. This difference may be due to less glycosylation sites in the mouse sequence.

Poids moléculaire: 58 kDa