ARHGAP5 anticorps (AA 1102-1214)

N° du produit ABIN968611

Cet anticorps Souris Monoclonal détecte spécifiquement ARHGAP5 dans WB et IF. Il présente une réactivité envers Humain, Rat et Chien et a été mentionné dans 2+ publications.

Aperçu rapide pour ARHGAP5 anticorps (AA 1102-1214) (ABIN968611)

Antigène: ARHGAP5 (rho GTPase Activating Protein 5 (ARHGAP5))

Reactivité: Humain, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ARHGAP5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 54-P190

Détail du produit anti-ARHGAP5 anticorps

Épitope: AA 1102-1214

Réactivité croisée: Rat (Rattus), Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human p190-B aa. 1102-1214

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ARHGAP5 (ABIN968611)

Burbelo, Finegold, Kozak, Yamada, Takami: "Cloning, genomic organization and chromosomal assignment of the mouse p190-B gene." dans: Biochimica et biophysica acta, Vol. 1443, Issue 1-2, pp. 203-10, (1999) (PubMed).

Burbelo, Miyamoto, Utani, Brill, Yamada, Hall, Yamada: "p190-B, a new member of the Rho GAP family, and Rho are induced to cluster after integrin cross-linking." dans: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 30919-26, (1996) (PubMed).

Détails sur ARHGAP5

Antigène: ARHGAP5 (rho GTPase Activating Protein 5 (ARHGAP5))

Autre désignation: p190-B

Sujet: GTPase activating proteins (GAPs) stimulate the GTP-hydrolyzing activity of GTPases, such as p21ras and Rho. p190-A is a Ras-GAP associated protein that is tyrosine phosphorylated in transformed and growth factor-stimulated cells. Ras-GAP and p190-A are targets of oncoproteins and growth factor receptors. p190-B is another Ras-GAP in the p190 family. It has 51% identity with p190-A and contains several GTPase-related domains in the N-terminal region and a Rho-GAP domain in the C-terminal region. p190-B is expressed in kidney, brain, liver, and lung, as well as in human foreskin fibroblasts, RD muscle cells, and HT-1080 cells. In fibroblasts, p190-B is localized diffusely in the cytoplasm and co-localizes with the alpha5beta1 integrin receptor for fibronectin. Adhesion of fibronectin-coated latex beads to cells leads to the recruitment of p190-B and Rho to the plasma membrane at sites of bead contact. In addition, the recombinant Rho-GAP domain of p190-B displays GAP activity for RhoA, Rac1, and G25K/CDC42Hs. Thus, p190-B is thought to act as a transmembrane link between integrins and Rho GTPases during fibronectin-induced changes in cell morphology and motility.

Poids moléculaire: 190 kDa

Pathways: Regulation of Cell Size