Espin anticorps (AA 458-580)

N° du produit ABIN968627

Détail du produit anti-Espin anticorps

Antigène: Espin (ESPN)

Épitope: AA 458-580

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Espin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Humain, Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat espin aa. 458-580

Clone: 31-Espin

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Espin (ABIN968627)

Chen, Li, Wang, Wang, Zheng, Bartles: "Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque." dans: Molecular biology of the cell, Vol. 10, Issue 12, pp. 4327-39, (2000) (PubMed).

Bartles, Zheng, Li, Wierda, Chen: "Small espin: a third actin-bundling protein and potential forked protein ortholog in brush border microvilli." dans: The Journal of cell biology, Vol. 143, Issue 1, pp. 107-19, (1998) (PubMed).

Bartles, Wierda, Zheng: "Identification and characterization of espin, an actin-binding protein localized to the F-actin-rich junctional plaques of Sertoli cell ectoplasmic specializations." dans: Journal of cell science, Vol. 109 ( Pt 6), pp. 1229-39, (1997) (PubMed).

Détails sur Espin

Antigène: Espin (ESPN)

Autre désignation: Espin (ESPN Produits)

Synonymes: anticorps DFNB36, anticorps je, anticorps Je, anticorps espin, anticorps ESPN, anticorps TVAG_100530, anticorps LOAG_01035, anticorps espn, anticorps Espn

Sujet: Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles. Espins are actin binding and bundling proteins. The two isoforms of espin are the 30 kDa small espin found in brush border cells and the 110 kDa espin found in testis. Espin contains eight ankyrin repeats in the N-terminal region, two proline-rich peptides, an ATP/GTP binding P-loop domain, and a C-terminal actin bundling domain. Small espin is composed of the C-terminal actin bundling domain and a unique region at the N-terminus. Espin binds to actin with a higher affinity than small espin and is more efficient at actin bundling. During spermiogenesis, espin accumulates, along with forming parallel actin bundles, at the ectoplasmic specialization. These actin bundles anchor and position the spermatid within the seminiferous epithelium. Other actin binding proteins, such as alpha-actinin, vinculin, and fimbrin, have also been implicated in the formation of ectoplasmic specialization, however espin appears to function specifically in the testes.

Poids moléculaire: 110 kDa

Pathways: Sensory Perception of Sound