PSIP1 anticorps (AA 85-188)

N° du produit ABIN968640

Cet anticorps anti-PSIP1 est un anticorps Souris Monoclonal détectant PSIP1 dans WB, IF et BI. Adapté pour Humain. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour PSIP1 anticorps (AA 85-188) (ABIN968640)

Antigène: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PSIP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Clone: 26-LEDGF

Détail du produit anti-PSIP1 anticorps

Épitope: AA 85-188

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human LEDGF aa. 85-188

Isotype: IgG1

Information d'application

Indications d'application: Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-PSIP1 (ABIN968640)

Cherepanov, Maertens, Proost, Devreese, Van Beeumen, Engelborghs, De Clercq, Debyser: "HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells." dans: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 372-81, (2002) (PubMed).

Nakamura, Singh, Kubo, Chylack, Shinohara: "LEDGF: survival of embryonic chick retinal photoreceptor cells." dans: Investigative ophthalmology & visual science, Vol. 41, Issue 5, pp. 1168-75, (2000) (PubMed).

Singh, Kimura, Chylack, Shinohara: "Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing." dans: Gene, Vol. 242, Issue 1-2, pp. 265-73, (2000) (PubMed).

Ge, Si, Wolffe: "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2." dans: Molecular cell, Vol. 2, Issue 6, pp. 751-9, (1999) (PubMed).

Ge, Si, Roeder: "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation." dans: The EMBO journal, Vol. 17, Issue 22, pp. 6723-9, (1999) (PubMed).

Détails sur PSIP1

Antigène: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Autre désignation: LEDGF

Sujet: P52/p75 proteins were first identified through their interaction with transcriptional factors and splicing factors. p75 is encoded by exons 1-15 and contains 530 amino acids, while the alternatively spliced p52 is encoded by exons 1-9 and contains 333 amino acids. Both p52 and p75 have strong interactions with the VP16 activation domain and with various components of the general transcriptional machinery. p52 is the more active transcription activator and has also been shown to recruit the pre-mRNA splicing factor ASF/SF to sites of active transcription. Interestingly, p52/p75 has also been identified as Lens Epithelium-Derived Growth Factor (LEDGF). In serum free media, LEDGF stimulates the growth and survival of a variety of cells, such as lens epithelial cells, retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes. In retinal photoreceptor cells, LEDGF is found in the nucleus where it promotes resistance to serum starvation and thermal stress. Thus, the LEDGF gene encodes splice variants that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival.
Synonyms: Lens Epithelium-Derived Growth Factor

Poids moléculaire: 75 kDa and/or 52 kDa

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization