F11R anticorps (AA 126-237)

N° du produit ABIN968747

L’anticorps Souris Monoclonal anti-F11R a été validé pour WB et IF. Il convient pour détecter F11R dans des échantillons de Humain. Il y a 4+ publications disponibles.

Aperçu rapide pour F11R anticorps (AA 126-237) (ABIN968747)

Antigène: F11R (F11 Receptor (F11R))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp F11R est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 43-JAM

Détail du produit anti-F11R anticorps

Épitope: AA 126-237

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human JAM aa. 126-237

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-F11R (ABIN968747)

Gupta, Pillarisetti, Ohlstein: "Platelet agonist F11 receptor is a member of the immunoglobulin superfamily and identical with junctional adhesion molecule (JAM): regulation of expression in human endothelial cells and macrophages." dans: IUBMB life, Vol. 50, Issue 1, pp. 51-6, (2001) (PubMed).

Ozaki, Ishii, Arai, Horiuchi, Kawamoto, Suzuki, Kita: "Junctional adhesion molecule (JAM) is phosphorylated by protein kinase C upon platelet activation." dans: Biochemical and biophysical research communications, Vol. 276, Issue 3, pp. 873-8, (2000) (PubMed).

Ozaki, Ishii, Horiuchi, Arai, Kawamoto, Okawa, Iwamatsu, Kita: "Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 2, pp. 553-7, (1999) (PubMed).

Martìn-Padura, Lostaglio, Schneemann, Williams, Romano, Fruscella, Panzeri, Stoppacciaro, Ruco, Villa, Simmons, Dejana: "Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration." dans: The Journal of cell biology, Vol. 142, Issue 1, pp. 117-27, (1998) (PubMed).

Détails sur F11R

Antigène: F11R (F11 Receptor (F11R))

Autre désignation: JAM-1

Sujet: Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intercellular barriers. Protein components of the tight junctions include actin filaments, symplekin, occludin, ZO-1, ZO-2, and ZO-3. Junctional adhesion molecules, JAM-1, -2, and -3, are a new subfamily in the immunoglobulin (Ig) superfamily that colocalize with these components of tight junctions. JAM-1 was identified through a screen of antibodies that recognizes antigens located at cell-cell contacts. The structure of JAM-1 includes two V-type Ig domains in the extracellular region, two N-glycosylation sites, and a small C-terminal cytoplasmic region. JAM-1 protein is expressed at cell-cell junctions in endothelial and epithelial cells, and antibodies to JAM-1 inhibit spontaneous and chemokine-induced monocyte transmigration through an endothelial cell monolayer. In addition, TNFalpha and IFN-gamma cause redistribution of JAM-1 from intercellular junctions to the cytoplasm. JAM-1 also is the receptor for the platelet membrane protein, F11 antigen, and activation of platelets leads to PKC phosphorylation of JAM-1. Thus, JAM-1 is a junctional adhesion molecule that functions in endothelial, epithelial, and platelet adhesion.

Poids moléculaire: 38 kDa

Pathways: Cell-Cell Junction Organization