GAP43 anticorps (AA 1-144)

N° du produit ABIN968792

Détail du produit anti-GAP43 anticorps

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Épitope: AA 1-144

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAP43 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human GAP-43/Neuromodulin aa. 1-144

Clone: 31-GAP

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-GAP43 (ABIN968792)

Chapman, Estep, Storm: "Palmitylation of neuromodulin (GAP-43) is not required for phosphorylation by protein kinase C." dans: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 25233-8, (1993) (PubMed).

Meiri, Bickerstaff, Schwob: "Monoclonal antibodies show that kinase C phosphorylation of GAP-43 during axonogenesis is both spatially and temporally restricted in vivo." dans: The Journal of cell biology, Vol. 112, Issue 5, pp. 991-1005, (1991) (PubMed).

Strittmatter, Valenzuela, Kennedy, Neer, Fishman: "G0 is a major growth cone protein subject to regulation by GAP-43." dans: Nature, Vol. 344, Issue 6269, pp. 836-41, (1990) (PubMed).

Cimler, Andreasen, Andreasen, Storm: "P-57 is a neural specific calmodulin-binding protein." dans: The Journal of biological chemistry, Vol. 260, Issue 19, pp. 10784-8, (1985) (PubMed).

Détails sur GAP43

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Autre désignation: GAP-43/Neuromodulin (GAP43 Produits)

Synonymes: anticorps B-50, anticorps PP46, anticorps Basp2, anticorps GAP-43, anticorps b-50, anticorps gap-43, anticorps gap43b, anticorps pp46, anticorps cb310, anticorps gap, anticorps gap43, anticorps gap43a, anticorps growth associated protein 43, anticorps growth associated protein 43 L homeolog, anticorps growth associated protein 43 S homeolog, anticorps GAP43, anticorps Gap43, anticorps gap43.L, anticorps gap43, anticorps gap43.S

Sujet: Neuromodulin (GAP-43, B-50, F-1) is a neural-specific protein that represents about one percent of the total membrane protein in growth cones from developing rat brain. Growth cones are located at the distal tip of neuronal processes and are critical for transduction of extracellular signals into directed growth. Neuromodulin is attached to the membranes of the neuronal growth cones during development. In the adult, it is attached to the presynaptic membranes in discrete subsets of synapses. Neuromodulin can bind to calmodulin, as well as inhibit phosphatidylinositol phosphate kinase (PIP kinase) activity. It is also a prominent substrate of Protein Kinase C (PKC) and may mediate some of the effects of PKC on growth cone function. As may be expected, expression and phosphorylation of neuromodulin are closely associated with axonal outgrowth and repair. Phosphorylation of neuromodulin by PKC has been correlated with the persistence of long term potentiation and the translocation of PKC to synaptosomal membranes. This PKC-mediated phosphorylation does not appear to require neuromodulin membrane association. It has also been demonstrated that the GTP-binding protein, G[o], is a main component of growth cone membranes. The binding of GTP--S to G[o] is stimulated by neuromodulin and this activity resides within the amino-terminal region of the protein. This region of neuromodulin shares sequence homology with the cytoplasmic tails of G-linked transmembrane receptors, suggesting the presence of one binding site on G[o] for both neuromodulin and transmembrane receptors. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 43 kDa