ASAH1 anticorps (AA 88-182)

N° du produit ABIN968809

Cet anticorps Souris Monoclonal détecte spécifiquement ASAH1 dans WB et IF. Il présente une réactivité envers Humain et Chien et a été mentionné dans 3+ publications.

Aperçu rapide pour ASAH1 anticorps (AA 88-182) (ABIN968809)

Antigène: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ASAH1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 23-Acid Ceramidase

Détail du produit anti-ASAH1 anticorps

Épitope: AA 88-182

Réactivité croisée: Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Acid Ceramidase aa. 88-182

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-ASAH1 (ABIN968809)

Ferlinz, Kopal, Bernardo, Linke, Bar, Breiden, Neumann, Lang, Schuchman, Sandhoff: "Human acid ceramidase: processing, glycosylation, and lysosomal targeting." dans: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 35352-60, (2001) (PubMed).

Strelow, Bernardo, Adam-Klages, Linke, Sandhoff, Krönke, Adam: "Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death." dans: The Journal of experimental medicine, Vol. 192, Issue 5, pp. 601-12, (2000) (PubMed).

Bernardo, Hurwitz, Zenk, Desnick, Ferlinz, Schuchman, Sandhoff: "Purification, characterization, and biosynthesis of human acid ceramidase." dans: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11098-102, (1995) (PubMed).

Détails sur ASAH1

Antigène: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Autre désignation: Acid Ceramidase

Sujet: Ceramide is a sphingolipid that exhibits a wide variety of functions, including monocyte differentiation, apoptosis, neurite outgrowth, and Ca2+ transport. It also serves as the precursor of many sphingolipids and anchors these into the outer leaflet of the plasma membrane via hydrophobic interactions. Acid ceramidase is a lysosomal enzyme that was purified from human urine. It is synthesized as a 55kDa precursor protein, which is then processesed into the mature alpha-subunit (13kDa) and beta-subunit (40kDa). Acid ceramidase catalyzes the hydrolysis of ceramide into free fatty acid and sphingosine. Sphingosine, and its phosphorylated form, sphingosine-1-phosphate (SPP), have been shown to inhibit PKC activity and act as a second messenger in cell proliferation and differentiation. Acid ceramidase is also the cause of a lysosomal storage disorder known as Farber’s disease. This disease is characterized by an accumulation of ceramide in tissues, leading to swelling and pain of the joints and extremities, pulmonary insufficiency, and death at an early age. Thus, acid ceramidase is necessary in the hydrolysis of ceramide and is the cause of Farber’s disease.

Poids moléculaire: 13 kDa