BUB1B anticorps (AA 276-388)

N° du produit ABIN968854

Cet anticorps Souris Monoclonal détecte spécifiquement BUB1B dans WB et IF. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour BUB1B anticorps (AA 276-388) (ABIN968854)

Antigène: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BUB1B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 9-BUBR1

Détail du produit anti-BUB1B anticorps

Épitope: AA 276-388

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human BUBR1 aa. 276-388

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-BUB1B (ABIN968854)

Wu, Lan, Li, Wu, Weinstein, Sakamoto, Dai: "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase." dans: Oncogene, Vol. 19, Issue 40, pp. 4557-62, (2000) (PubMed).

Chan, Jablonski, Sudakin, Hittle, Yen: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." dans: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, (1999) (PubMed).

Mizunuma, Hirata, Miyahara, Tsuchiya, Miyakawa: "Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast." dans: Nature, Vol. 392, Issue 6673, pp. 303-6, (1998) (PubMed).

Détails sur BUB1B

Antigène: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Autre désignation: BUBR1

Sujet: Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately atttached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, Bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast Bub1 and Bub3 form a complex that binds kinetochores and has protein kinase activity. BUBR1 is a bub-related kinase homologous to Bub1 and MAD3. BUBR1 contains a nuclear localization signal and two highly conserved domains CD1 and CD2. CD1 is in the N-terminal region and is invoved in kinetochore localization, while CD2 is in the C-terminal region and is involved in kinase activity. BUBR1 is hyperphosphorylated during spindle checkpoint activation, and interacts with p55CDC and APC during spindle checkpoint function. In colorectal cancers, the BUBR1 gene has structural abnormalities that suggest BUBR1 is a tumor suppressor gene. Thus, BUBR1 may be a critical spindle checkpoint kinase and disruption of its function may be related to tumor formation.

Poids moléculaire: 125 kDa