Actin anticorps

N° du produit ABIN968903

L’anticorps Souris Monoclonal anti-Actin a été validé pour WB et IF. Il convient pour détecter Actin dans des échantillons de Humain, Rat, Souris, Poulet et Chien. Il y a 4+ publications disponibles.

Aperçu rapide pour Actin anticorps (ABIN968903)

Antigène: Actin

Reactivité: Humain, Rat, Souris, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: C4-actin

Détail du produit anti-Actin anticorps

Réactivité croisée: Humain, Chien, Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Chicken gizzard muscle Actin

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Actin (ABIN968903)

Yamamori, Itakura, Sugaya, Katsumata, Sakagami, Takahashi: "Differential expression of SNAP-25 family proteins in the mouse brain." dans: The Journal of comparative neurology, Vol. 519, Issue 5, pp. 916-32, (2011) (PubMed).

Pantaloni, Le Clainche, Carlier: "Mechanism of actin-based motility." dans: Science (New York, N.Y.), Vol. 292, Issue 5521, pp. 1502-6, (2001) (PubMed).

Mitchison, Cramer: "Actin-based cell motility and cell locomotion." dans: Cell, Vol. 84, Issue 3, pp. 371-9, (1996) (PubMed).

Hanstein, Lange, Schneider-Poetsch, Grolig, Wagner: "Detection of actin and localization of phytochrome in the green alga Mougeotia by monoclonal antibodies." dans: Acta histochemica. Supplementband, Vol. 41, Issue 9, pp. 223-30, (1992) (PubMed).

Détails sur Actin

Antigène: Actin

Sujet: Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments results from interactions between actin and actin-binding proteins. Actin is a 42-kDa protein that is known as G-actin in its monomeric form. Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin. F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks. The latter are highly concentrated beneath the plasma membrane and form the actin cortex. Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins. These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures. Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.

Poids moléculaire: 42 kDa