Nanog anticorps (AA 20-166)

N° du produit ABIN969308

L’anticorps Souris Monoclonal anti-Nanog a été validé pour WB, ELISA et ICC. Il convient pour détecter Nanog dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Nanog anticorps (AA 20-166) (ABIN969308)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: 1E6C4

Détail du produit anti-Nanog anticorps

Épitope: AA 20-166

Fonction: NANOG Antibody

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-Nanog (ABIN969308)

Freberg, Dahl, Timoskainen, Collas: "Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract." dans: Molecular biology of the cell, Vol. 18, Issue 5, pp. 1543-53, (2007) (PubMed).

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: NANOG

Sujet: NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52 % overall amino acid identity with the mouse protein and 85 % identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

Poids moléculaire: 35 kDa

ID gène: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance