FGA anticorps

N° du produit ABIN99199

N° du produit (Fournisseur): 100-101-240

Cet anticorps Chèvre Polyclonal détecte spécifiquement FGA dans WB, IHC et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour FGA anticorps (ABIN99199)

Antigène: FGA (Fibrinogen alpha Chain (FGA))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FGA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-FGA anticorps

N° du produit (Fournisseur): 100-101-240

Fournisseur: Rockland

Fonction: Fibrinogen Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fibrinogen [Human Plasma].

Attributs du produit: Synonyms: goat anti-Fibrinogen Antibody, FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Fibrinogen [Human Plasma]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Fibrinogen has been tested by western blot and immunohistochemistry and is suitable to be assayed against 1.0 μg of Fibrinogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur FGA

Antigène: FGA (Fibrinogen alpha Chain (FGA))

Autre désignation: FGA

Sujet: Background: Fibrinogen is cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA), fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, it functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. It was originally thought to be essential for platelet aggregation, based on in vitro studies using anti-coagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. It enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. It may also facilitate the immune response via both innate and T-cell mediated pathways.

ID gène: 2243

NCBI Accession: NP_000499

UniProt: P02671