GLB1 anticorps (FITC)

N° du produit ABIN99528

N° du produit (Fournisseur): 200-4236

Cet anticorps anti-GLB1 est un anticorps Lapin Polyclonal détectant GLB1 dans WB et FM. Adapté pour E. coli.

Aperçu rapide pour GLB1 anticorps (FITC) (ABIN99528)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLB1 est conjugé à/à la FITC

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Détail du produit anti-GLB1 anticorps (FITC)

N° du produit (Fournisseur): 200-4236

Fournisseur: Rockland

Fonction: Beta Galactosidase Antibody Fluorescein Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Beta Galactosidase (E.

Attributs du produit: Synonyms: rabbit anti-Beta Galactosidase Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal FITC conjugated antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Beta Galactosidase (E. coli)

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 3.5

Information d'application

Indications d'application:

Application Note: Anti-Beta Galactosidase Fluorescein Conjugated Antibody has been tested by western blot and is designed for fluorescent western blotting, also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Western Blot Dilution: 1:10,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur GLB1

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Beta Galactosidase

Sujet: Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

NCBI Accession: NP_414878

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process