GLB1 anticorps (HRP)

N° du produit ABIN99529

Détail du produit anti-GLB1 anticorps (HRP)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLB1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Labeling (Lbl), Fluorescence Microscopy (FM), Conjugation (Con)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: Beta Galactosidase (E.coli)
Immunogentype:Native

Isotype: IgG

Information d'application

Indications d'application: Suitable for immunoblotting (western or dot blot), ELISA, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.  A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.  A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.  Although a wide range of conditions was reported to be effective, a 1:10,000 dilution was noted to show no background and to be suitable for double labeling experiments.  Optimal titers for other applications should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Do NOT add Sodium Azide!

Stock: 4 °C

Détails sur GLB1

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Beta Galactosidase (GLB1 Produits)

Synonymes: anticorps EBP, anticorps ELNR1, anticorps MPS4B, anticorps DER, anticorps XR, anticorps 0610038K04Rik, anticorps 1810027P18Rik, anticorps glb, anticorps CG9092, anticorps Dmel\\CG9092, anticorps beta-GAL, anticorps beta-Gal-1, anticorps beta-gal, anticorps gal, anticorps lacZ-1, anticorps AW125515, anticorps Bge, anticorps Bgl, anticorps Bgl-e, anticorps Bgl-s, anticorps Bgl-t, anticorps Bgs, anticorps Bgt, anticorps C130097A14Rik, anticorps BGAL, anticorps zgc:110823, anticorps ECK0341, anticorps JW0335, anticorps GLB, anticorps galactosidase beta 1, anticorps dicarbonyl and L-xylulose reductase, anticorps dicarbonyl L-xylulose reductase, anticorps beta-D-galactosidase, anticorps beta-galactosidase, anticorps beta galactosidase, anticorps galactosidase, beta 1, anticorps beta-galactosidase-like, anticorps GLB1, anticorps DCXR, anticorps Dcxr, anticorps lacZ, anticorps SSP_RS00525, anticorps lacH, anticorps ECL_03691, anticorps Gal, anticorps Glb1, anticorps glb1, anticorps VV2_1327, anticorps LOC100056371

Sujet: Synonyms: HRP

Pathways: Glycosaminoglycan Metabolic Process