Glycerol Kinase anticorps

N° du produit ABIN99889

N° du produit (Fournisseur): 100-101-211

Cet anticorps Chèvre Polyclonal détecte spécifiquement Glycerol Kinase dans ELISA et WB. Il présente une réactivité envers Cellulomonas.

Aperçu rapide pour Glycerol Kinase anticorps (ABIN99889)

Antigène: Glycerol Kinase (GK)

Reactivité: Cellulomonas

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Glycerol Kinase est non-conjugé

Application: ELISA, Western Blotting (WB)

Détail du produit anti-Glycerol Kinase anticorps

N° du produit (Fournisseur): 100-101-211

Fournisseur: Rockland

Fonction: Glycerol Kinase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glycerol Kinase [Cellulomonas species].

Attributs du produit: Synonyms: goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Glycerol Kinase [Cellulomonas species]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Application Note: Anti-Glycerol Kinase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glycerol Kinase [Celullomonas species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Glycerol Kinase

Antigène: Glycerol Kinase (GK)

Autre désignation: glpK

Sujet: Background: Glycerol Kinase is the key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It is inhibited by fructose 1,6-bisphosphate (FBP). This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.

ID gène: 6960048

UniProt: D0VZG4